PXD058160

PXD058160 is an original dataset announced via ProteomeXchange.

Title	Expanding the Landscape of Aging via Orbitrap Astral Mass Spectrometry and Tandem Mass Tag (TMT) Integration
Description	Aging results in progressive decline in physiological function due to the deterioration of essential biological processes, such as transcription and RNA splicing, ultimately increasing mortality risk. Although proteomics is emerging as a crucial tool for elucidating the molecular underpinnings of aging, existing studies are constrained by limited proteome coverage and only observe a narrow range of lifespan. To overcome these limitations, we employed the Orbitrap Astral Mass Spectrometer with the multiplex tandem mass tag (TMT) technology to profile the proteomes of three brain tissues (cortex, hippocampus, striatum) and kidney in the C57BL/6 mouse model, achieving high-resolution quantification, ranging from 8,954 to 9,376 proteins per tissue. Our sample population represents balanced sampling across both sexes and three age groups (3, 12, and 20 months), comprising approximately young adulthood to early late life (~20-60 years-old for human lifespan). To enhance quantitative accuracy, we developed a rigorous peptide filtering strategy based on resolution and signal-to-noise thresholds. Our analysis uncovered distinct tissue-specific patterns of protein abundance, with pronounced age and sex differences in the kidney, contrasted by limited sex differences in brain tissues. Notably, we identified both continuous and non-continuous age-associated proteomic changes, revealing complex protein dynamics over adult lifespan. Integrating our findings with early developmental proteomic data from brain tissues highlighted further divergent age-related trajectories, particularly in synaptic proteins. This study not only expands the proteomic landscape of aging, but also underscores the utility of Orbitrap Astral Mass Spectrometry for capturing age-associated molecular alterations with unprecedented depth.
HostingRepository	PRIDE
AnnounceDate	2025-04-07
AnnouncementXML	Submission_2025-04-07_01:50:31.198.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058160
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tian Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-21 19:48:07	ID requested
1	2025-04-03 12:46:33	announced
⏵ 2	2025-04-07 01:50:31	announced	2025-04-07: Updated project metadata.

10.6019/PXD058160;

submitter keyword: Tandem Mass Tag(TMT), proteomics, agining, mouse,Orbitrap Astral mass spectrometer

Tian Zhang
contact affiliation	Department of Biochemistry and Molecular Genetics University of Virginia School of Medicine
contact email	cgm8ck@virginia.edu
lab head
Tian Zhang
contact affiliation	University of Virginia
contact email	CGM8CK@VIRGINIA.EDU
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058160
     1. Label: PRIDE project
     2. Name: Expanding the Landscape of Aging via Orbitrap Astral Mass Spectrometry and Tandem Mass Tag (TMT) Integration