PXD058139 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | SUMOylation study in mascRNA-transfected cells |
| Description | We aimed to characterize changes within the SUMOylation pathway that are driven by the tRNA-like molecule mascRNA. For the experiments otulined below, we used 293FT cells (a derivative of HEK293T cells).
First, we identified the SAE2-associating proteins. Sample IDs: 92072 NRS control, 92073 antiSAE2 IP.
Secondly, we identified proteins pulled-down by biotinylated mascRNA and mascRNA-3'CCA, using scrambled mascRNA oligo as a control. Sample IDs: 92199: scrambled mascRNA-Biotin control; 92200: mascRNA3'biotinTEG; 92201: mascRNA-CCA3'biotinTEG
Thirdly, we verified the specificity of SUMO1 IPs, by comparing two beads elution approaches:
1) by SUMO1 competitive peptide elution (processed gel pieces). Sample IDS: 99699-99704: Proteomic analysis of SDS PAGE gel pieces for SUMO1 IP and control IgG IP. (Details below)
2) urea eluates from the SUMO1-IPs. Sample IDs: 99705 control Rabbit IgG IP; 99706 SUMO1 IP
Lastly, we used urea eluates from SUMO1-IPs from cells transfected with 5’P-mascRNA vs. 5’Pscrambled RNA. Sample IDs:
100479, 100480, 100481: Triplicates (1A, 1B, 1C) of control IgG IP from cells treated with scrambled mascRNA oligo (control IP)
100482, 100483, 100484: Triplicates (3A, 3B, 3C) of control IgG IP from cells treated with mascRNA oligo (control IP)
100485, 100486, 100487: Triplicates (2A, 2B, 2C) of SUMO1 IP from cells treated with scrambled mascRNA oligo (control transfection)
100488, 100489, 100490: Triplicates (4A, 4B, 4C) of SUMO1 IP from cells treated with mascRNA oligo
Sample details can be found in the file named Experimental_Design_Proteomics_ELIAPI_2024.txt below. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-20 |
| AnnouncementXML | Submission_2026-01-19_16:36:25.222.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Eleni Liapi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-11-21 08:40:50 | ID requested | |
| ⏵ 1 | 2026-01-19 16:36:25 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
Contact List
| Anastasios Vourekas |
|---|
| contact affiliation | Anastasios Vourekas 131 Life Science Life Building Department of Biological Sciences Louisiana State University Baton Rouge, LA 70803 office at A108 (225-578-1737) lab at A151-A149 (225-578-9103) |
| contact email | avourekas@lsu.edu |
| lab head | |
| Eleni Liapi |
|---|
| contact affiliation | Louisiana State University |
| contact email | eliapi1@lsu.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058139
- Label: PRIDE project
- Name: SUMOylation study in mascRNA-transfected cells
