PXD058133

PXD058133 is an original dataset announced via ProteomeXchange.

Title	Structural basis of RNA polymerase complexes in African swine fever virus
Description	African swine fever virus is highly contagious and causes a fatal infectious disease in pigs, resulting in a significant global impact on pork supply. The African swine fever virus RNA polymerase serves as a crucial multifunctional protein complex responsible for genome transcription and regulation. Therefore, it is essential to investigate its structural and functional characteristics for the prevention and control of African swine fever. Here, we determine the structures of endogenous African swine fever virus RNA polymerase in both nucleic acid-free and elongation states. The African swine fever virus RNA polymerase shares similarities with the core of typical RNA polymerases, but possesses a distinct subunit M1249L. Notably, the dynamic binding mode of M1249L with RNA polymerase, along with the C-terminal tail insertion of M1249L in the active center of DNA-RNA scaffold binding, suggests the potential of M1249L to regulate RNA polymerase activity within cells. These results are important for understanding the transcription cycle of African swine fever virus and for developing antiviral strategies.
HostingRepository	PRIDE
AnnounceDate	2025-02-14
AnnouncementXML	Submission_2025-02-14_06:56:45.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	吴瑕 曾
SpeciesList	scientific name: African swine fever virus; NCBI TaxID: 10497;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-21 04:48:29	ID requested
⏵ 1	2025-02-14 06:56:46	announced

10.1038/s41467-024-55683-z;

Zhu G, Xi F, Zeng W, Zhao Y, Cao W, Liu C, Yang F, Ru Y, Xiao S, Zhang S, Liu H, Tian H, Yang F, Lu B, Sun S, Song H, Sun B, Zhao X, Tang L, Li K, He J, Guo J, Zhu Y, Zhu Z, Sun F, Zheng H, Structural basis of RNA polymerase complexes in African swine fever virus. Nat Commun, 16(1):501(2025) [pubmed]

submitter keyword: protein,African swine fever virus,LC-MSMS

Guoliang Zhu
contact affiliation	Lanzhou University
contact email	zhugl@lzu.edu.cn
lab head
吴瑕 曾
contact affiliation	兰州大学
contact email	2572557201@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD058133

PRIDE project URI

• PRIDE
  1. PXD058133
     1. Label: PRIDE project
     2. Name: Structural basis of RNA polymerase complexes in African swine fever virus