PXD058121

PXD058121 is an original dataset announced via ProteomeXchange.

Title	Tracking Proteasome Degradation: A Cross-Organ Analysis via Intact degradomics Top-Down Mass Spectrometry
Description	Identifying the peptides generated by the proteasome is essential for understanding its role in regulating critical cellular processes such as apoptosis, signaling, and immune responses. In this study, we introduce an enzymatic top-down mass spectrometry approach that allows real-time monitoring of proteasome-mediated cleavage, providing detailed insights into substrate degradation patterns. By examining proteasomes from different organs, we reveal organ-specific peptide production and degradation behaviors, offering a deeper understanding of how proteasome subtypes affect cellular function. This method is well-suited for investigating the influence of proteasome regulators, inhibitors and activators, on proteasome activity. Additionally, its versatility makes it applicable to other proteolytic complexes, paving the way for further research on proteostasis systems.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:06:48.656.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katharina Zittlau
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive UHMR

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-21 00:04:08	ID requested
⏵ 1	2025-08-24 16:06:49	announced

Zittlau KI, Zachor-Movshovitz D, Leushkin Y, Schimmel Brener R, Morgenstern D, Ben-Nissan G, Sharon M, Tracking proteasome degradation: A cross-organ analysis via intact degradomics mass spectrometry. Proc Natl Acad Sci U S A, 122(8):e2419607122(2025) [pubmed]

10.1073/pnas.2419607122;

submitter keyword: mass spectrometry, top-down MS, proteasome,protein degradation

Michal Sharon
contact affiliation	Department of Biomolecular Sciences, Weizmann Institute of Science
contact email	michal.sharon@weizmann.ac.il
lab head
Katharina Zittlau
contact affiliation	Weizmann Institute of Science
contact email	katharina.zittlau@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD058121

PRIDE project URI

• PRIDE
  1. PXD058121
     1. Label: PRIDE project
     2. Name: Tracking Proteasome Degradation: A Cross-Organ Analysis via Intact degradomics Top-Down Mass Spectrometry