PXD058096

PXD058096 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomic Analysis of Dental-Origin Stem Cells: Insights into Regenerative Potential
Description	Teeth are a significant source of stem cells and have clinical importance for regenerative medicine. A human tooth harbors different kinds of stem cells in the dental pulp (DPSC) or the periodontal ligament (PDLSC). Also exfoliated teeth in childhood contain a special type of stem cells in their pulp called Stem cells from Human Exfoliated Deciduous teeth (SHED). All these stem cells have features and capacities that vary depending on their niche. Evaluating dental-originated stem cells via molecular analysis tools is important to gain insight into their regenerative potential. Here we investigated the proteomic properties of three types of stem cells that originated from human teeth. We isolated and cultured the DPSCs, PDLSCs, and SHED cells. After validating MSC populations via immunophenotyping, we performed a mass spectrometry-based proteomic approach to identify and relatively quantify whole cell and secreted proteins. Identified proteins were evaluated by using Gene Ontology and Reactome pathway analysis tools. Our data reveal that SHED cells represented inflammation, hypoxia, and nutrient deficiency-associated ontologies in both their secretome and whole-cell proteomes. The whole-cell proteome of PDLSCs consisted of differentiation and proliferation-associated molecules while their secretory molecules were mainly associated with inflammation, ECM organization, and immune response ontologies. Among dental-originated stem cells, DPSCs appeared to be the healthiest and clinically relevant in terms of proteomic properties with their proliferation, growth factor signaling, and stemness-associated pathways and ontologies in their secretome and whole-cell proteome. Obtained results demonstrated that every type of stem cell from dental origin has unique proteomic features that are altered by their location and physiological conditions. Considering these findings may help researchers improve the dental stem-cell-based regenerative medicine approaches.
HostingRepository	PRIDE
AnnounceDate	2026-04-07
AnnouncementXML	Submission_2026-04-07_08:17:12.855.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Servet ÖZCAN
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	alkylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-20 09:07:26	ID requested
⏵ 1	2026-04-07 08:17:13	announced

10.1007/s12015-025-10936-2;

Tez B Ç, Durukan SM, Y, ı, ld, ı, r SK, Ç, okke, ç, eci M, Boyvat D, Alt, ı, nsoy N, F, ı, nd, ı, k F, G, ü, ner Ş A, Acar MB, Galderisi U, Ö, zcan S, Comparative Proteomic Analysis of Dental-Origin Stem Cells: Insights into Regenerative Potential. Stem Cell Rev Rep, 21(7):2287-2297(2025) [pubmed]

submitter keyword: Proteome, PDLSC,DPSC, Secretome, SHED

Servet Özcan
contact affiliation	Erciyes University Genome and Stem Cell Center (GENKOK)
contact email	ozcan@erciyes.edu.tr
lab head
Servet ÖZCAN
contact affiliation	Erciyes University Genkök
contact email	ozcan@erciyes.edu.tr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058096
     1. Label: PRIDE project
     2. Name: Comparative Proteomic Analysis of Dental-Origin Stem Cells: Insights into Regenerative Potential