PXD058084

PXD058084 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profile at Revision Arthroplasty for Periprosthetic Joint Infection Correlates with Disease Stage and Surgical Outcome
Description	Periprosthetic joint infection (PJI) is both the most common and difficult to treat form of arthroplasty failure, occurring in ~2% of primary total knee and hip arthroplasties. For patient and surgeon alike, treating PJI with DAIR surgery (debridement, antibiotics, and implant retention) is preferable to implant resection surgery due to medical costs, patient morbidity, and required skill, however, DAIR success rates are variable. Therefore, an accurate diagnostic to identify candidates who will undergo DAIR successfully would be clinically helpful. To achieve this, advanced proteomic analysis of sonicate fluid from 95 subjects with PJI was performed using proximity extension assay (PEA), with 77 samples additionally undergoing liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis. A group of 120 proteins was identified that successfully separates DAIR samples into success or failure categories and predicts DAIR surgery outcome post-hoc. Additionally, the proteomic signature associated with short symptom duration and long symptom duration PJI was defined and used to categorize samples into 2 putative stages of infection. The investigation of 7,261 unique human proteins was employed here to explore the physiology of PJI and reveals a proteomic signature for DAIR failure as well as insight into PJI protein profiles based on patient reported symptom duration.
HostingRepository	PRIDE
AnnounceDate	2025-08-27
AnnouncementXML	Submission_2025-08-26_17:22:50.182.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-20 04:10:18	ID requested
⏵ 1	2025-08-26 17:22:50	announced

Dataset with its publication pending

submitter keyword: DAIR, Prosthetic joint infection,Sonicate fluid, Proteomics

Akhilesh Pandey
contact affiliation	Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA
contact email	pandey.akhilesh@mayo.edu
lab head
Akhilesh Pandey
contact affiliation	Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905
contact email	pandey.akhilesh@mayo.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058084
     1. Label: PRIDE project
     2. Name: Proteomic Profile at Revision Arthroplasty for Periprosthetic Joint Infection Correlates with Disease Stage and Surgical Outcome