PXD058082

PXD058082 is an original dataset announced via ProteomeXchange.

Title	Major toxin composition of particulate matter from a coal-fired power plant and its proteomics-associated toxicological profiles in the lungs of balb/c mice
Description	Coal-fired power plant is a major method of electricity generation worldwide with serious concerns relating to air pollution. In recent years, fly ash from coal-fired power plants has become a major target of pollution control by worldwide regulatory agencies. In this present study, fly ash particulate matter (PM) obtained from the electrostatic precipitator of Mae Moh Coal-fired Power Plant (Lampang, Thailand) was selected as a model for determination of physicochemical characteristics and toxicological profiles in mice. The average size of the fly ash PM was 4.29 ± 1.85 µm as determined by electron microscopy. Major heavy metal was iron at 101,067.31 ± 8,246.12 mg/kg as detected by ICPMS. Acenaphthene was the most abundant polyaromatic hydrocarbon at 40.77 ± 0.003 mg/kg as analyzed by GCMS. The toxicological profiles of the characterized fly ash PM were conducted in Balb/c mice by intratracheal instillation at 1 and 10 mg/kg/day for 7 days, compared to vehicle as negative control and standard PM 2.5 as positive control. The fly ash PM and the standard PM induced significant pulmonary inflammation in mice lungs compared to vehicle control. In addition, molecular toxicology of mice lungs investigated by proteomics demonstrated 3 inflammation associated pathways, which include leukocyte transendothelial migration, phagosome, and neutrophil extracellular trap formation. Furthermore, only high dose (10 mg/kg/day) standard PM and high dose fly ash PM showed high expression of NCF2, which potentially promotes cancer in the long run. This study is the first to report on the physicochemical characterization, histopathology, and proteomic-associated toxicological profiles of the fly ash PM obtained from the largest coal-fired power plant in Thailand. Information obtained from this study is useful for academic researchers, electricity enterprises, and policymakers to develop appropriate strategies to manage air pollution from coal-fired power plants worldwide.
HostingRepository	PRIDE
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_12:33:07.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meng Chieh Yang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-20 01:34:29	ID requested
⏵ 1	2025-08-21 12:33:07	announced

10.1016/J.TOXREP.2025.102110;

submitter keyword: particulate matter, polyaromatic hydrocarbons, proteomics, coal-fired power plant, heavy metals, pulmonary inflammation

Arnatchai Maiuthed
contact affiliation	Centre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
contact email	arnatchai.mai@mahidol.ac.th
lab head
Meng Chieh Yang
contact affiliation	Centre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University
contact email	mengchiehyang99@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD058082

PRIDE project URI

• PRIDE
  1. PXD058082
     1. Label: PRIDE project
     2. Name: Major toxin composition of particulate matter from a coal-fired power plant and its proteomics-associated toxicological profiles in the lungs of balb/c mice