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PXD058056

PXD058056 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInteraction of saposin C and acid-beta-glucosidase
DescriptionThe objective of this project was to determine the interaction interface(s) between acid beta-glucosidase (GCase) and its activator protein saposin C (SapC). Chemical crosslinking was used to confirm the formation of discrete heterodimers between the two proteins, using lysine-reactive crosslinkers BS3 and DTSSP and both non-activating and activating buffer systems. High-resolution mass spectrometry was then used to determine the sites of crosslinking, which informed the modes of protein-protein interactions. We identified two binding surfaces between GCase and saposin C using this workflow.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_04:57:47.244.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMinh Thu Ma
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF; Orbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-11-19 14:41:53ID requested
12025-05-07 04:57:48announced
Publication List
Dobert JP, Sch, ä, fer JH, Dal Maso T, Ravindran P, Huard DJE, Socher E, Schildmeyer LA, Lieberman RL, Vers, é, es W, Moeller A, Zunke F, Arnold P, -glucocerebrosidase in complex with its transporter LIMP-2. Nat Commun, 16(1):3074(2025) [pubmed]
10.1038/s41467-025-58340-1;
Keyword List
submitter keyword: Gaucher, lysosome, glucosidase, saposin, crosslinking
Contact List
Raquel Lieberman
contact affiliationSchool of Chemistry & Biochemistry, Georgia Institute of Technology
contact emailraquel.lieberman@chemistry.gatech.edu
lab head
Minh Thu Ma
contact affiliationDana-Farber Cancer Institute
contact emailminh_ma@dfci.harvard.edu
dataset submitter
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Dataset FTP location
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