PXD058056 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Interaction of saposin C and acid-beta-glucosidase |
Description | The objective of this project was to determine the interaction interface(s) between acid beta-glucosidase (GCase) and its activator protein saposin C (SapC). Chemical crosslinking was used to confirm the formation of discrete heterodimers between the two proteins, using lysine-reactive crosslinkers BS3 and DTSSP and both non-activating and activating buffer systems. High-resolution mass spectrometry was then used to determine the sites of crosslinking, which informed the modes of protein-protein interactions. We identified two binding surfaces between GCase and saposin C using this workflow. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_04:57:47.244.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Minh Thu Ma |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-11-19 14:41:53 | ID requested | |
⏵ 1 | 2025-05-07 04:57:48 | announced | |
Publication List
Dobert JP, Sch, ä, fer JH, Dal Maso T, Ravindran P, Huard DJE, Socher E, Schildmeyer LA, Lieberman RL, Vers, é, es W, Moeller A, Zunke F, Arnold P, -glucocerebrosidase in complex with its transporter LIMP-2. Nat Commun, 16(1):3074(2025) [pubmed] |
10.1038/s41467-025-58340-1; |
Keyword List
submitter keyword: Gaucher, lysosome, glucosidase, saposin, crosslinking |
Contact List
Raquel Lieberman |
contact affiliation | School of Chemistry & Biochemistry, Georgia Institute of Technology |
contact email | raquel.lieberman@chemistry.gatech.edu |
lab head | |
Minh Thu Ma |
contact affiliation | Dana-Farber Cancer Institute |
contact email | minh_ma@dfci.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058056 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058056
- Label: PRIDE project
- Name: Interaction of saposin C and acid-beta-glucosidase