PXD058056

PXD058056 is an original dataset announced via ProteomeXchange.

Title	Interaction of saposin C and acid-beta-glucosidase
Description	The objective of this project was to determine the interaction interface(s) between acid beta-glucosidase (GCase) and its activator protein saposin C (SapC). Chemical crosslinking was used to confirm the formation of discrete heterodimers between the two proteins, using lysine-reactive crosslinkers BS3 and DTSSP and both non-activating and activating buffer systems. High-resolution mass spectrometry was then used to determine the sites of crosslinking, which informed the modes of protein-protein interactions. We identified two binding surfaces between GCase and saposin C using this workflow.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_04:57:47.244.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minh Thu Ma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-19 14:41:53	ID requested
⏵ 1	2025-05-07 04:57:48	announced

Dobert JP, Sch, ä, fer JH, Dal Maso T, Ravindran P, Huard DJE, Socher E, Schildmeyer LA, Lieberman RL, Vers, é, es W, Moeller A, Zunke F, Arnold P, -glucocerebrosidase in complex with its transporter LIMP-2. Nat Commun, 16(1):3074(2025) [pubmed]

10.1038/s41467-025-58340-1;

submitter keyword: Gaucher, lysosome, glucosidase, saposin, crosslinking

Raquel Lieberman
contact affiliation	School of Chemistry & Biochemistry, Georgia Institute of Technology
contact email	raquel.lieberman@chemistry.gatech.edu
lab head
Minh Thu Ma
contact affiliation	Dana-Farber Cancer Institute
contact email	minh_ma@dfci.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058056

PRIDE project URI

• PRIDE
  1. PXD058056
     1. Label: PRIDE project
     2. Name: Interaction of saposin C and acid-beta-glucosidase