PXD058048

PXD058048 is an original dataset announced via ProteomeXchange.

Title	The effect of citrate supplementation on medium viscosity and poly-γ-glutamic acid synthesis by engineered B. subtilis 168
Description	The industrially attractive biopolymer Poly-γ-glutamic acid (γ-PGA) is commonly produced by species of the genus Bacillus by co-feeding different carbon- and nitrogen sources. Recent studies have highlighted the pivotal role of co-metabolization of a rapidly degradable carbon source such as glycerol together with citrate for γ-PGA production, independently fueling biomass generation as well as TCA cycle precursor supply. With this study, we report that the sole presence of citrate in the production medium greatly influences growth behavior, γ-PGA production, and the viscosity of microbial cultures during biopolymer synthesis. Independent of the citrate concentration in the medium. only minor amounts of citrate were imported by B. subtilis 168 in presence of glycerol due to carbon catabolite repression. However, a high citrate concentration resulted in a 6-fold increase in γ-PGA titer as compared to low levels of exogenous citrate. Data suggests that citrate was not used as a precursor in γ-PGA synthesis but rather influenced the fate of imported glutamate. The citrate concentration also affected medium viscosity as depletion resulted in a remarkable spike in broth viscosity. Additionally, cellular proteome analysis at different levels of citrate availability revealed significant changes in protein abundance involved in motility and fatty acid degradation
HostingRepository	PRIDE
AnnounceDate	2025-02-10
AnnouncementXML	Submission_2025-02-10_04:55:59.174.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-19 07:40:42	ID requested
⏵ 1	2025-02-10 04:55:59	announced

10.1002/ELSC.70009;

submitter keyword: ViMOS (Viscosity Monitoring Online System), γ-PGA, viscosity, catabolite repression,biopolymer, Bacillus subtilis, co-feed

Dörte Becher
contact affiliation	Department of Microbial Proteomics, University of Greifswald, Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058048
     1. Label: PRIDE project
     2. Name: The effect of citrate supplementation on medium viscosity and poly-γ-glutamic acid synthesis by engineered B. subtilis 168