PXD058027 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of BEAS-2B lung epithelial cells after the introduction of the BRAF V600E mutation using CRISPR/Cas9 |
Description | To mimic the naturally occurring BRAF V600E mutation in lung cancer, a BRAF V600E knock-in BEAS-2B cell model was established using CRISPR/Cas9. The proteomics analysis was carried out to evaluate the underlying changes at the proteome level. Proteomics analysis revealed significant changes in the proteins involved in the biological processes including epithelial-mesenchymal transition (EMT), extracellular matrix (ECM)-receptor interaction, cell adhesion, focal adhesion, and cell metabolism upon BRAF V600E mutation. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-22 |
AnnouncementXML | Submission_2025-04-22_02:02:15.653.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lingyun Dai |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-11-19 00:21:36 | ID requested | |
⏵ 1 | 2025-04-22 02:02:16 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), China Human Proteome Project (CNHPP), Human Proteome Project |
submitter keyword: V600E, Lung, amino acid metabolism, NSCLC,BRAF |
Contact List
Lingyun Dai |
contact affiliation | School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, China |
contact email | lingyun.dai@outlook.com |
lab head | |
Lingyun Dai |
contact affiliation | School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, China |
contact email | lingyun.dai@outlook.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD058027 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058027
- Label: PRIDE project
- Name: Proteomic analysis of BEAS-2B lung epithelial cells after the introduction of the BRAF V600E mutation using CRISPR/Cas9