PXD058017

PXD058017 is an original dataset announced via ProteomeXchange.

Title	E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells
Description	The E2 ubiquitin (Ub)-conjugating enzyme primarily determines Ub conjugation as Ub-isopeptide (lysine), Ub-oxyester (serine/threonine) or Ub-thioester (cysteine). However, E2-specific Ub conjugation profiles within cells remain elusive. Here, we developed the fusion E2–Ub-R74G profiling (FUSEP) strategy to access E2-specific Ub conjugation profiles in cells with amino acid resolution. The probe-specific leucine-glycine-glycine-glycine-modified Ub remnant enables systematic studies of nonlysine Ub conjugation and provides site-specific information. Multiple E2 enzymes were found to be involved in nonlysine ubiquitination. Profiling with UBE2D3–Ub-R74G probes identified the existence of tyrosine ubiquitination in human Cullin-1, a scaffold protein for Cullin-RING E3 Ub ligases. This modification is distinct from lysine ubiquitination. A single-pass membrane-bound E3 ligase, RNF149, was identified to pair with UBE2D3 to regulate pyroptosis by ubiquitinating apoptosis-associated speck-like protein ASC. The availability of this toolbox paves the way for uncovering E2-specific Ub conjugation profiles and identifying previously unknown E3 Ub ligases for potential therapeutic applications.
HostingRepository	iProX
AnnounceDate	2024-12-11
AnnouncementXML	Submission_2024-12-10_22:56:30.590.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Siqi Shen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-18 19:06:18	ID requested
⏵ 1	2024-12-10 22:56:30	announced

Dataset with its publication pending

submitter keyword: chemical tools, ubiquitin probes, protein post-translational modifications

Hang Yin
contact affiliation	Tsinghua University
contact email	yin_hang@tsinghua.edu.cn
lab head
Siqi Shen
contact affiliation	Tsinghua University
contact email	sqshen@tsinghua.edu.cn
dataset submitter

iProX dataset URI