PXD058011

PXD058011 is an original dataset announced via ProteomeXchange.

Title	An insight into the body louse, Pediculus humanus (Frisco strain), salivary gland contents.
Description	Human body lice, Pediculus humanushumanus, are blood-feeding parasites that live in human clothing and feed several times per day.Saliva from louse bites induces local inflammation in the skin, pruiritis,and, if untreated, chronic Pediculosis has systemic negative health effects.Despite the well-established medical importance of body lice and their longstanding coevolution with humans, characterization of their saliva has been very limited.To address this, we extracted RNA and protein from two of the body louse’s morphologically distinct, sets of salivary glands (Bean-shaped and U-shaped) and generated transcript and protein profiles for each.Additionally, we performed fluorescent staining and confocal microscopy on each gland type to enhance descriptions of their morphology and detail their gross cellular architecture and arrangement.Analysis of the body louse salivary gene products and proteins revealed that the overwhelming majority were not closely related to biomolecules of known function; highlighting the organism’s unique and understudied saliva composition.Unexpectedly, and despite the contrasting morphology of the 2 gland types, there was ahigh degree of overlap in the salivary products produced.This finding suggests a strongDarwinian selection pressure to maintain both salivary glands types, given that it would seemingly be simpler to have a single morphologically homogeneous set of salivary glands.Here, we present the first next-generation sequencing and proteomic characterization of the human body louse sialome, discuss the potential physiological importance of louse salivary proteins, and present possible explanations for why lice have such a complex salivary gland organization despite ahigh degree of redundancy in protein repertoires between Bean and U-shaped salivary glands.
HostingRepository	PRIDE
AnnounceDate	2025-02-19
AnnouncementXML	Submission_2025-02-19_09:46:43.781.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephen Lu
SpeciesList	scientific name: Pediculus humanus corporis; NCBI TaxID: 121224;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-18 14:16:39	ID requested
⏵ 1	2025-02-19 09:46:44	announced

Dataset with its publication pending

submitter keyword: RNA sequencing, transcriptome, vector, salivary gland, hematophagous arthropods, proteome, sialome

Stephen Lu
contact affiliation	Vector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.
contact email	stephen.lu@nih.gov
lab head
Stephen Lu
contact affiliation	NIH/NIAID
contact email	stephen.lu@nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058011
     1. Label: PRIDE project
     2. Name: An insight into the body louse, Pediculus humanus (Frisco strain), salivary gland contents.