PXD057996

PXD057996 is an original dataset announced via ProteomeXchange.

Title	Map of the Neuronal Tissue O-glycoproteome Reveal Specific Traits in the Secretogranin Family
Description	Impairments in protein glycosylation cause severe developmental disorders and most with prominent neurological involvement. This includes the most abundant GalNAc-type O-glycosylation, but unravelling underlying disease etiologies caused by deficiencies in this glycosylation pathway is hampered by limited knowledge of neuronal O-glycoproteins. Here we report a comprehensive map of GalNAc-type O-glycoproteins (>800) and O-glycosites (>4,000) from neuronal tissues and cell lines that are compiled in a web-based resource. We identified abundant O-glycosites within major classes of proteins involved in neuroplasticity, including perineuronal nets, synapse formation, axon guidance, membrane remodeling and dense core granulogenesis. We demonstrate that Chromogranin A, a key player in neurotransmitter signaling, is abundantly decorated with O-glycans and glycosaminoglycans, and that glycosylation is important for proper multimerization. We further demonstrate that neuronal cell lines deficient in O-glycosylation exhibit higher capacity for storing the neurotransmitter noradrenaline and enlarged neurotransmitter-containing dense core granules
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-27_18:13:07.808.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergey Vakhrushev
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-18 09:13:38	ID requested
⏵ 1	2025-07-27 18:13:08	announced

10.1016/j.jbc.2025.110313;

Madsen TD, Topaktas AB, Dworkin LA, Hintze J, Hansen LH, Nikpour M, Lackman JJ, Goth CK, Larson G, Shiplett EJ, Edmondson AC, Zhou Z, Miller RL, Joshi HJ, Vakhrushev SY, Schjoldager KT, Map of the neuronal O-glycoproteome reveals driver functions in the regulated secretory pathway. J Biol Chem, 301(7):110313(2025) [pubmed]

submitter keyword: glycosylation, neurotransmitter,Granin, mucin, neuron, perineuronal net (PNN), glycosaminoglycans, central nervous system (CNS), neuroplasticity, dense core granules (DCG)

Sergey Vakhrushev
contact affiliation	Department of Cellular and Molecular Medicine, University of Copenhagen
contact email	seva@sund.ku.dk
lab head
Sergey Vakhrushev
contact affiliation	Department of Cellular and Molecular Medicine
contact email	seva@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD057996

PRIDE project URI

• PRIDE
  1. PXD057996
     1. Label: PRIDE project
     2. Name: Map of the Neuronal Tissue O-glycoproteome Reveal Specific Traits in the Secretogranin Family