PXD057991

PXD057991 is an original dataset announced via ProteomeXchange.

Title	Identification of a Zinc Carboxypeptidase in a crude lysate SDS-PAGE gel of a Porphyromonas gingivalis transcription regulator mutant
Description	Porphyromonas gingivalis is one of the main etiological agents responsible for the initiation and progression of periodontal diseases. This bacterium inhabits unfavorable conditions, therefore, to adapt to the changing external environment, e.g. redox conditions, and nutrient limitations, it must respond quickly to these stress factors. For this purpose, it uses transcription regulators. One of them can be the CrpPg protein classified to the CRP/FNR protein superfamily. This study aimed to analyze the properties of the CrpPg protein and identify the processes it regulates in the context of P. gingivalis virulence. Inactivating the crpPg gene did not affect bacterial growth in liquid culture. However, the absence of CrpPg reduced the bacterium's ability to form biofilm while increasing its adhesion to and invasion of gingival keratinocytes. SDS-PAGE analysis of lysates from both the P. gingivalis ATCC 33277 wild-type strain and its corresponding ΔcrpPg mutant revealed a key difference: in the ΔcrpPg mutant, a ~120 kDa band disappeared while a ~100 kDa band intensified compared to the wild-type strain. Mass spectrometry confirmed that both the ~100 kDa and ~120 kDa bands correspond to a putative zinc carboxypeptidase encoded by the PG0232 gene. Furthermore, both the A7436 strain and its ΔcrpPg mutant form produce only the ~100 kDa variant of this zinc carboxypeptidase. Our study shows that CrpPg can be important for the precise regulation of genes encoding virulence factors required to invade host cells and regulate bacterial replication inside host cells to evade the host’s immune response.
HostingRepository	PRIDE
AnnounceDate	2025-03-27
AnnouncementXML	Submission_2025-03-27_06:10:11.884.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057991
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michał Tracz
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Porphyromonas gingivalis ATCC 33277; NCBI TaxID: 431947;
ModificationList	monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-18 08:03:32	ID requested
⏵ 1	2025-03-27 06:10:12	announced

10.3389/FCIMB.2025.1535009;

10.6019/PXD057991;

submitter keyword: MSE,LC-MS, SDS-PAGE, CRP/FNR protein superfamily, gel band analysis, transcription regulator mutant, putative zinc carboxypeptidase, bottom-up, Porphyromonas gingivalis

Michał Tracz
contact affiliation	Laboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
lab head
Michał Tracz
contact affiliation	Laboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057991
     1. Label: PRIDE project
     2. Name: Identification of a Zinc Carboxypeptidase in a crude lysate SDS-PAGE gel of a Porphyromonas gingivalis transcription regulator mutant