PXD057987

PXD057987 is an original dataset announced via ProteomeXchange.

Title	The nuclear sulfenome of Arabidopsis: spotlight on histone acetyltransferase GCN5 regulation through functional thiols
Description	In aerobic life forms, reactive oxygen species (ROS) are produced by the partial reduction of oxygen during energy-generating metabolic processes. In plants, ROS production increases during periods of both abiotic and biotic stress, severely overloading the antioxidant systems. Hydrogen peroxide (H2O2) plays a central role in cellular redox homeostasis and signaling by oxidising crucial cysteines to sulfenic acid, which is considered a biologically relevant post-translational modification (PTM). Until now, the impact of the nucleus on cellular redox homeostasis has been relatively unexplored. The regulation of histone-modifying enzymes by oxidative PTMs at redox-sensitive cysteine or tyrosine residues is particularly intriguing because it allows the integration of redox signaling mechanisms with chromatin control of transcriptional activity. One of the most extensively studied histone acetyltransferases is the conserved GENERAL CONTROL NONDEPRESSIBLE 5 (GCN5) complex. This study investigated the nuclear sulfenome in Arabidopsis thaliana by expressing a nuclear variant of the Yeast Activation Protein-1 (YAP1) probe and identified 225 potential redox-active proteins undergoing S-sulfenylation. Mass spectrometry analysis further confirmed the S-sulfenylation of GCN5 at cysteines 293, 368, and 400, and their functional significance and impact on the GCN5 protein-protein interaction network were assessed using cysteine-to-serine mutagenesis.
HostingRepository	PRIDE
AnnounceDate	2025-02-20
AnnouncementXML	Submission_2025-02-20_02:46:30.414.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057987
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Frank Van Breusegem
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	N-methylmaleimide modified L-cysteine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-18 06:17:54	ID requested
⏵ 1	2025-02-20 02:46:31	announced

De Smet B, Yang X, Plskova Z, Castell C, Fern, á, ndez-Fern, á, ndez A, Dard A, Masood J, Mhamdi A, Huang J, Vertommen D, Chan KX, Pyr Dit Ruys S, Messens J, Kerchev PI, Van Breusegem F, The nuclear sulfenome of Arabidopsis: spotlight on histone acetyltransferase GCN5 regulation through functional thiols. J Exp Bot, 76(6):1569-1584(2025) [pubmed]

10.1093/jxb/erae514;

10.6019/PXD057987;

submitter keyword: sulfenylation,Arabidopsis, GCN5, dimedone

Frank Van Breusegem
contact affiliation	UGent Department of Plant Biotechnology and Bioinformatics VIB Center of Plant Systems Biology
contact email	frank.vanbreusegem@ugent.be
lab head
Frank Van Breusegem
contact affiliation	UGent Department of Plant Biotechnology and Bioinformatics VIB Center of Plant Systems Biology
contact email	frank.vanbreusegem@ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057987
     1. Label: PRIDE project
     2. Name: The nuclear sulfenome of Arabidopsis: spotlight on histone acetyltransferase GCN5 regulation through functional thiols