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PXD057968

PXD057968 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInvestigating Neutrophil Responses to Stimuli: Comparative Analysis of reactive species- dependent and independent mechanisms
DescriptionNeutrophils play a critical role in immune response, using mechanisms as degranulation, and release of neutrophil extracellular traps (NETs) to combat pathogens. NETs are web-like structures composed of chromatin and microbicidal proteins, released by neutrophils in response to many different stimuli. Recent evidences suggest multiple mechanisms might be involved in neutrophil’s response to stimuli, but the biochemical characterization of each different pathway is still lacking. In this study, we used superoxide measurements, live-imaging microscopy and high-resolution proteomics to provide a careful biochemical characterization of the neutrophil’s response following activation by two commonly used, well characterized stimuli, namely phorbol-12-myristate-13-acetate (PMA) , and ionomycin, a calcium ionophore. Our results demonstrated that although both stimuli induce extracellular DNA release, signals and mediators released by activated cells before this final event were distinct. Thus, PMA-treated neutrophils induce superoxide production, and degranulation of proteins from all granules, especially those derived from secretory vesicles and tertiary granules. On the other hand, ionomycin-treated neutrophils do not stimulate superoxide generation, but induce extensive protein citrullination particularly in the proteins related to actin cytoskeleton organization, nucleus stability, and the NADPH oxidase complex. Interestingly, many of the citrullinated proteins detected in this work were also found to act as autoantigens in autoimmune diseases such as rheumatoid arthritis. These striking differences show neutrophils’ response to PMA and ionomycin are two distinct biochemical processes that might exemplify distinct response to the environment. It also provides implications for understanding neutrophil-driven microbial response and potential roles in autoimmune diseases.
HostingRepositoryPRIDE
AnnounceDate2025-03-03
AnnouncementXMLSubmission_2025-03-03_05:28:38.100.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRafaela Nascimento
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-11-17 11:25:52ID requested
12025-03-03 05:28:38announced
Publication List
10.1016/J.REDOX.2025.103540;
Keyword List
submitter keyword: degranulation,Neutrophil extracellular traps, ionomycin, NADPH oxidase activation, PMA
Contact List
Graziella Eliza Ronsein
contact affiliationDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil
contact emailronsein@iq.usp.br
lab head
Rafaela Nascimento
contact affiliationUniversity of São Paulo - Department of Biochemistry
contact emailrnascimento@iq.usp.br
dataset submitter
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Dataset FTP location
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