PXD057954

PXD057954 is an original dataset announced via ProteomeXchange.

Title	Reactive oxygen species control protein degradation at the mitochondrial import gate
Description	While reactive oxygen species (ROS) have long been known to drive aging and neurodegenera-tion, their persistent depletion below basal levels also disrupts organismal function. Cells counter-act loss of basal ROS via the reductive stress response, but the identity and biochemical activity of ROS sensed by this pathway remain unknown. Here, we show that the reductive stress E3 ligase CUL2FEM1B specifically acts at mitochondrial TOM complexes, where it is regulated by ROS that are produced as byproducts of complex III of the electron transport chain (ETC) during oxidative phosphorylation. By modulating localized CUL2FEM1B activity, ROS tune protein import into mitochondria and thereby control the abundance of the rate-limiting ETC complex IV. As complex III yields most ROS when the ETC outpaces metabolic demands or oxygen availability, our findings identify basal ROS as sentinels of mitochondrial activity that help cells adjust their ETC to fluctuating environments, as required for cell differentiation and survival.
HostingRepository	PRIDE
AnnounceDate	2024-12-05
AnnouncementXML	Submission_2024-12-05_14:17:51.068.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057954
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rachael McMinimy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ XL

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-15 19:38:25	ID requested
⏵ 1	2024-12-05 14:17:51	announced

10.1016/J.MOLCEL.2024.11.004;

10.6019/PXD057954;

submitter keyword: TOM complex, mitochondria, ubiquitin

Michael Rapé
contact affiliation	UC Berkeley MCB department
contact email	mrape@berkeley.edu
lab head
Rachael McMinimy
contact affiliation	UC Berkeley
contact email	rmcminimy@berkeley.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD057954

PRIDE project URI

• PRIDE
  1. PXD057954
     1. Label: PRIDE project
     2. Name: Reactive oxygen species control protein degradation at the mitochondrial import gate