PXD057948

PXD057948 is an original dataset announced via ProteomeXchange.

Title	Exploration of Artificial Intelligence-Based Spectral Libraries for Data Independent Acquisition in Complex Ocean Metaproteomic Analyses
Description	Ocean metaproteomics provides valuable insights into the structure and function of marine microbial communities. Yet, ocean samples are challenging due to their extensive biological diversity that results in a very large number of peptides with a large dynamic range. This study characterized the capabilities of data independent acquisition (DIA) mode for use in ocean metaproteomic samples. Spectral libraries were constructed from discovered peptides and proteins using machine learning algorithms to remove incorporation of false positives in the libraries. When compared with 1-dimensional and 2-dimensional data dependent acquisition analyses (DDA), DIA outperformed DDA both with and without gas phase fractionation. We found that larger discovered protein spectral libraries performed better, regardless of the geographic distance between where samples were collected for library generation and where the test samples were collected. Moreover, the spectral library containing all unique proteins present in the Ocean Protein Portal outperformed smaller libraries generated from individual sampling campaigns. However, a spectral library constructed from all open reading frames in a metagenome was found to be too large to be workable, resulting in low peptide identifications due to challenges maintaining a low false discovery rate with such a large database size. Given sufficient sequencing depth and validation studies, spectral libraries generated from previously discovered proteins can serve as a community resource, saving resequencing efforts. The spectral libraries generated in this study are available at the Ocean Protein Portal for this purpose.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-29_14:48:51.994.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew McIlvin
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: environmental samples
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-15 11:17:47	ID requested
⏵ 1	2025-09-29 14:48:52	announced

10.22541/AU.173218889.93373224/V1;

submitter keyword: DDA DIA Ocean metaproteomics proteomics

Mak Saito
contact affiliation	Woods Hole Oceanographic Institution
contact email	msaito@whoi.edu
lab head
Matthew McIlvin
contact affiliation	Woods Hole Oceanographic Inst.
contact email	mmcilvin@whoi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057948
     1. Label: PRIDE project
     2. Name: Exploration of Artificial Intelligence-Based Spectral Libraries for Data Independent Acquisition in Complex Ocean Metaproteomic Analyses