PXD057942

PXD057942 is an original dataset announced via ProteomeXchange.

Title	Trichodesmium metaproteome samples across three ocean regions
Description	The diazotroph Trichodesmium is an important contributor to marine dinitrogen (N2) fixation, supplying so-called new N to phytoplankton in typically N-limited ocean regions. Identifying how iron (Fe) and phosphorus (P) influence Trichodesmium activity and biogeography is an ongoing area of study, where predicting patterns of resource stress is complicated in part by the uncertain bioavailability of organically complexed Fe and P. Here, a comparison of 26 metaproteomes from picked Trichodesmium colonies identified significantly different patterns between three ocean regions: the western tropical South Pacific, the western North Atlantic, and the North Pacific Subtropical Gyre. Trichodesmium metaproteomes across these regions significantly differed in KEGG submodule signals, and vector fitting showed that dissolved Fe, phosphate, and temperature significantly correlated with regional proteome patterns. Populations in the western tropical South Pacific appeared to modulate their proteomes in response to both Fe and P stress, including a comparatively low relative abundance of the N2 fixation marker protein, NifH. Significant increases in the relative abundance of both Fe and P stress marker proteins previously validated in culture studies suggested that Trichodesmium populations in the western North Atlantic and North Pacific were P-stressed and Fe-stressed, respectively. These patterns recapitulate established regional serial and co-limitation patterns of resource stress on phytoplankton communities. Evaluating community stress patterns may therefore predict resource controls on diazotroph biogeography. These data highlight how Trichodesmium modulates its metabolism in the field and provide an opportunity to more accurately constrain controls on Trichodesmium biogeography and N2 fixation.
HostingRepository	PRIDE
AnnounceDate	2025-06-17
AnnouncementXML	Submission_2025-06-17_09:15:29.197.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057942
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hanna Anderson
SpeciesList	scientific name: Trichodesmium sp.; NCBI TaxID: 1207;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-15 08:45:25	ID requested
⏵ 1	2025-06-17 09:15:29	announced

10.1093/ISMEJO/WRAF120;

10.6019/PXD057942;

submitter keyword: co-limitation, metaproteome,Trichodemium, marker

Sonya T
contact affiliation	Department of Earth and Environmental Science, Columbia University; Lamont-Doherty Earth Observatory, Columbia University
contact email	sdyhrman@ldeo.columbia.edu
lab head
Hanna Anderson
contact affiliation	Columbia University
contact email	ha2606@columbia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD057942

PRIDE project URI

• PRIDE
  1. PXD057942
     1. Label: PRIDE project
     2. Name: Trichodesmium metaproteome samples across three ocean regions