PXD057937

PXD057937 is an original dataset announced via ProteomeXchange.

Title	The proteome of osteoblasts in a 3D culture bioreactor model with static and perfusion conditions using biomimicry
Description	Disorders influencing bone form a major burden worldwide, with osteoporosis being the most common fracture-inducing bone disease. Currently, animal models are being utilized to develop and screen novel treatments. However, due to interspecies variations, different microenvironments, and ethical considerations, there is a need for a complex 3D bone model. This research aimed to perform a pilot study on developing a standardized representative bone model using a perfusion-based bioreactor, and to characterize the overall 3D bone proteomic profile. An immortalized human fetal osteoblastic cell line (hFOB1.19) was seeded on collagen scaffolds and cultured for 21 days in a perfusion bioreactor system and in static cultures. Different analyses allowed the monitoring of cell viability and compared morphological and proteome differences between both conditions. Scanning electron microscopy images suggested an altered cellular morphology within the bioreactor culture compared to the static cultures. A proteomic analysis identified a total of 1897 proteins and a total of 93 unique regulated proteins. Enrichment analyses of these proteins revealed seven significant pathways including TNF-alpha signaling pathway via NF-kB. These pathways also included hypoxia and epithelial-mesenchymal transition, where the EMT pathway was the most significant and indicated a reduction in cell motility within the bioreactor system on day 21. This preliminary study suggests that the U-CUP perfusion bioreactor is promising for further exploration of facilitating osteogenic differentiation induction in 3D cultures.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:02:59.673.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akos Vegvari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-15 07:17:37	ID requested
⏵ 1	2025-05-07 05:03:00	announced

Radi S, EzEldeen M, V, é, gv, á, ri Á, Coates D, Jacobs R, Bostanci N, Bao K, The proteome of osteoblasts in a 3D culture perfusion bioreactor model compared with static conditions. Sci Rep, 15(1):12120(2025) [pubmed]

10.1038/s41598-025-96632-0;

submitter keyword: human fetal osteoblastic cells, proteomics, and regenerative medicine, bioreactor,bone, tissue engineering

Akos Vegvari
contact affiliation	Karolinska Institutet, Stockholm, Sweden
contact email	akos.vegvari@ki.se
lab head
Akos Vegvari
contact affiliation	Karolinska Institutet
contact email	akos.vegvari@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057937
     1. Label: PRIDE project
     2. Name: The proteome of osteoblasts in a 3D culture bioreactor model with static and perfusion conditions using biomimicry