PXD057911

PXD057911 is an original dataset announced via ProteomeXchange.

Title	Plasma Exosomes in Insulin Resistant Obesity Exacerbate Progression of Triple Negative Breast Cancer
Description	Breast cancer, the most common cancer among women worldwide, continues to pose significant public health challenges. Among the subtypes of breast cancer, triple-negative breast cancer (TNBC) is particularly aggressive and difficult to treat due to the absence of receptors for estrogen, progesterone, or human epidermal growth factor receptor 2, rendering TNBC refractory to conventional targeted therapies. Emerging research underscores the exacerbating role of metabolic disorders, such as type 2 diabetes and obesity, on TNBC aggressiveness. Here, we investigate the critical cellular and molecular factors underlying this link. We explore the pivotal role of circulating plasma exosomes in modulating the tumor microenvironment and enhancing TNBC aggressiveness. We find that plasma exosomes from diet-induced obesity mice induce epithelial-mesenchymal transition features in TNBC cells, leading to increased migration in vitro and enhanced metastasis in vivo. We build on our previous reports demonstrating that plasma exosomes from obese, diabetic patients, and exosomes from insulin-resistant 3T3-L1 adipocytes, upregulate key transcriptional signatures of epithelial-mesenchymal transition in breast cancer. Bioinformatic analysis reveals that TNBC cells exhibit higher expression and activation of proteins related to the Rho-GTPase cascade, particularly the small Ras-related protein Rac1. Our approach suggests novel therapeutic targets and exosomal biomarkers, ultimately to improve prognosis for TNBC patients with co-morbid metabolic disorders.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:38:36.411.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stanley Goldstein
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-14 19:43:35	ID requested
⏵ 1	2025-07-13 16:38:37	announced

Ll, é, venes P, Chen A, Lawton M, Rond, ó, n-Ortiz AN, Qiu Y, Seen M, Monti S, Denis GV, Plasma exosomes in insulin resistant obesity exacerbate progression of triple negative breast cancer. BMC Cancer, 25(1):1089(2025) [pubmed]

10.1186/s12885-025-14447-8;

submitter keyword: Insulin Resistance,Exosomes, Obesity, Triple Negative Breast Cancer

Gerald Denis
contact affiliation	Boston University School of Medicine
contact email	pabloll@bu.edu
lab head
Stanley Goldstein
contact affiliation	Boston University
contact email	stangold@bu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057911
     1. Label: PRIDE project
     2. Name: Plasma Exosomes in Insulin Resistant Obesity Exacerbate Progression of Triple Negative Breast Cancer