PXD057893
PXD057893 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Distinguishing N-Terminal Methylation from Near-isobaric Modifications by Statistical Analysis of Mass Error Distributions of Fragment Ions |
| Description | alpha-N-terminal methylation is an understudied post-translational modification with presumed functions involving protein-protein and/or protein-DNA interaction. The covalent addition of mono-, di-, trimethyl groups to free alpha-amino group has proven to be difficult to profile globally by mass spectrometry due to trace endogenous amount and interference from near-isobaric modifications such as Nt-acetylation, even after N-terminome enrichment. To address this problem, we assume that in each MS2 spectrum b-ions will have a different mass error distribution compared to y-ions if the spectrum is falsely assigned to near-isobaric Nt-modification and exploit this statistically to correct the Nt-modification, a procedure we name as mass error test (MET) |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-05-22 |
| AnnouncementXML | Submission_2025-05-22_21:22:01.988.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Hankyul Lee |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Acetyl; Trimethyl; Formyl; Dimethyl; Methyl; Acetyl:2H(3); Oxidation; Carbamidomethyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-11-14 05:38:05 | ID requested | |
| ⏵ 1 | 2025-05-22 21:22:02 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: N-terminal methylation, mass error test, post-translational modification, mass error distribution, near isobaric modification, DatasetType:Proteomics |
Contact List
| Cheolju lee | |
|---|---|
| contact affiliation | Korea Institute of Science and Technology |
| contact email | clee270@kist.re.kr |
| lab head | |
| Hankyul Lee | |
| contact affiliation | KIST |
| contact email | dlgksruf321@kist.re.kr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000096415/ |
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