PXD057890

PXD057890 is an original dataset announced via ProteomeXchange.

Title	Redefined complement C3c structures have significant increase in the plasma of ovarian cancer patients
Description	Ovarian cancer (OC) ranks as the third most common gynecological cancer. Like other cancers, there still a need for effective biomarkers. To explore if plasma proteolytic products could serve as biomarkers, we devised a platform incorporating CyDye labeling, macroporous reversed-phase liquid chromatography, reducing/non-reducing SDS-PAGE, and fluorescence imaging. With paired preoperative and postoperative plasma samples, our analyses revealed a circulating complement C3 derivative present only at the disease state. This 145-kDa species under non-reducing condition split into 72-, 39-, and 29-kDa fragments upon reduction, reminiscent of the C3c structure. Mass spectrometric analyses showed multiple C-terminal ends in the C3c α’1 fragment, which were used differently among OC patients. The variety of ends were also observed in serum samples prepared using various complement activators, redefining C3c as a mix of multiple molecular entities. A commercialized ELISA assay targeting only the canonical C3c showed a strong correlation between increased plasma levels and the OC occurrence and pro-gression. Our results suggest that plasma proteolysis in complement deactivation is involved in ovarian tumorigenesis, and the resulting protein changes may help develop next-generation cancer biomarker.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_19:16:59.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong-Hao Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-14 03:51:53	ID requested
⏵ 1	2025-05-25 19:17:00	announced

10.1097/jcma.0000000000001249;

Chen DH, Chang YW, Chien CH, Torng PL, Tsay YG, Redefined complement C3c structures have significant increase in the plasma of ovarian cancer patients. J Chin Med Assoc, ():(2025) [pubmed]

submitter keyword: ovarian cancer, mass spectrometry, biomarker discovery, complement component C3, plasam proteolysis

Yeou-Guang Tsay
contact affiliation	Program in Molecular Medicine, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan
contact email	tsayyeou@nycu.edu.tw
lab head
Dong-Hao Chen
contact affiliation	Program in Molecular Medicine, National Yang-Ming Chiao Tung University and Academia Sinica, Taipei, Taiwan
contact email	kissshot.ls08@nycu.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD057890

PRIDE project URI

• PRIDE
  1. PXD057890
     1. Label: PRIDE project
     2. Name: Redefined complement C3c structures have significant increase in the plasma of ovarian cancer patients