PXD057852
PXD057852 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of site-specific O- and N-glycopeptides from recombinant spike and ACE2 glycoproteins using LC-MS/MS analysis |
| Description | The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in millions of infections and deaths globally. Although vaccination campaigns are mitigating the pandemic, emerging viral variants continue to pose challenges. The spike (S) protein of SARS-CoV-2 plays a critical role in viral entry by binding to the angiotensin-converting enzyme 2 (ACE2) receptor, making both proteins essential targets for therapeutic and vaccine development. Glycosylation of these proteins influences their structure and function, underscoring the need for detailed site-specific glycoproteomic analysis. In this study, we characterized the N- or O-glycosylation profiles of recombinant receptor-binding domain (RBD) of spike protein and ACE2 proteins expressed from HEK293 cells, as well as S2 subunit of spike protein expressed in plants cells. Using a high-resolution Orbitrap Eclipse Tribrid mass spectrometer integrated with the Ultimate 3000 RSLCnano and IQ-GPA (Identification and Quantification of GlycoProteome Analyzer), 148 N- and 28 O-glycopeptides from RBD, 71 N-glycopeptides from the S2 subunit, and 139 N-glycopeptides from ACE2 were characterized. Additionally, we report novel post-translational modifications (PTMs) in RBD glycopeptides, including mannose-6-phosphate (M6P) and GlcNAc-1-phosphate-6-O-mannose of N-glycan, and O-acetylation of O-glycan, identified for the first time. These findings provide valuable insights into the glycosylation patterns that influence protein function and immunogenicity, offering new perspectives for the development of vaccines and antibody-based therapies against COVID-19. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-11-14 |
| AnnouncementXML | Submission_2024-11-14_23:37:39.030.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ju Yeon Lee |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: SARS coronavirus; NCBI TaxID: 227859; |
| ModificationList | Glycosyl |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-11-13 09:10:15 | ID requested | |
| ⏵ 1 | 2024-11-14 23:37:39 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Spike glycoprotein, Reaction Binding Domain, Recombinant proteins, site-specific glycopeptides, DatasetType:Proteomics |
Contact List
| Ju Yeon Lee | |
|---|---|
| contact affiliation | Korea Basic Science Institute |
| contact email | jylee@kbsi.re.kr |
| lab head | |
| Ju Yeon Lee | |
| contact affiliation | KBSI |
| contact email | jylee@kbsi.re.kr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000096403/ |
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