PXD057839

PXD057839 is an original dataset announced via ProteomeXchange.

Title	Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells
Description	Mitosis is a critical phase of the cell cycle and a vulnerable point where cancer cells can be effectively disrupted, leading to cell death and inhibition of tumor growth. However, challenges such as drug resistance remain significant in clinical applications. During mitosis, mRNA translation is generally downregulated, while non-canonical translation of specific transcripts proceeds. Here, we demonstrate that mitotic cancer cells redistribute ribosomes toward the 5' untranslated region (5' UTR) and the start of the coding sequence (CDS), enhancing the translation of thousands of upstream open reading frames (uORFs) and upstream overlapping open reading frames (uoORFs). This mitotic induction of uORF/uoORF enriches the presentation of immunopeptides at the surface of cancer cells following treatment with mitotic inhibitors. Functional assays indicate the potential of such neoepitopes to provoke cancer-cell killing by T cells. Altogether, our findings highlight the therapeutic potential of targeting uORF/uoORF-derived neoepitopes in combination with mitotic inhibitors to enhance immune recognition and tumor cell elimination.
HostingRepository	PRIDE
AnnounceDate	2025-06-05
AnnouncementXML	Submission_2025-06-05_10:49:26.115.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonas P Becker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-13 05:04:03	ID requested
⏵ 1	2025-06-05 10:49:26	announced

Dataset with its publication pending

submitter keyword: FAIMS, DIA,uORF, immunopeptidomics, neoepitope

Fabricio Loayza-Puch
contact affiliation	Translational Control and Metabolism, German Cancer Research Center (DKFZ), Heidelberg, Germany
contact email	f.loayza-puch@dkfz-heidelberg.de
lab head
Jonas P Becker
contact affiliation	German Cancer Research Center (DKFZ)
contact email	jonas01.becker@dkfz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057839
     1. Label: PRIDE project
     2. Name: Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells