PXD057832

PXD057832 is an original dataset announced via ProteomeXchange.

Title	Shigella infection is facilitated by interaction of human enteric α-defensin 5 with colonic epithelial receptor P2Y11
Description	Human enteric α-defensin 5 (HD5) acts as a “double-edged sword” in host immunity due to its prominent role in promoting pathogen infections, yet the mechanistic basis of its infection-enhancing operation remains unclarified. Here we show that HD5 induces abundant filopodial extensions in epithelial cells which capture Shigella, a major human enteroinvasive pathogen that exploits filopodia for invasion, at high efficiency, suggesting a novel human-specific mechanism of HD5-augmented bacterial invasion. Using multi-omics screening and both gut-on-chip and in vivo models, we identify the HD5 receptor as P2Y11, a purinergic receptor distributed apically on the luminal surface of human colonic epithelium, which to our knowledge is the first HD5 receptor for a specific cellular function. cAMP-PKA signaling was identified to be the main pathway mediating the cytoskeleton-regulating activity of HD5, whose critical residues involved were also determined. In illuminating this mechanism of Shigella invasion, our findings raise the possibility of new intervention strategies against HD5-augmented infections.
HostingRepository	PRIDE
AnnounceDate	2024-12-16
AnnouncementXML	Submission_2024-12-15_22:36:51.179.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mengyao Guo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-13 00:35:47	ID requested
⏵ 1	2024-12-15 22:36:51	announced

10.1038/S41564-024-01901-9;

submitter keyword: Shigella

human defensin 5

filopodia

P2Y11

invasion

Dan Xu
contact affiliation	Xi'an Jiaotong University
contact email	dan.xu@xjtu.edu.cn
lab head
Mengyao Guo
contact affiliation	Xi'an Jiaotong University
contact email	myguo123@stu.xjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057832
     1. Label: PRIDE project
     2. Name: Shigella infection is facilitated by interaction of human enteric α-defensin 5 with colonic epithelial receptor P2Y11