PXD057813

PXD057813 is an original dataset announced via ProteomeXchange.

Title	SILAC of hESCs with DDX18 knockdown
Description	This study explores the crucial role of the nucleolus-specific RNA helicase DDX18 in maintaining the pluripotency of human embryonic stem cells. We demonstrate that DDX18 coordinates nucleolus phase separation and chromatin organization by interacting with NPM1 in the granular component of the nucleolus, facilitated by nucleolar RNA species. The absence of DDX18 disrupts immiscible nucleolar substructures, significantly affecting centromere clustering and perinucleolar heterochromatin (PNH) formation.
HostingRepository	PRIDE
AnnounceDate	2024-11-18
AnnouncementXML	Submission_2024-11-18_10:40:09.839.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-12 14:09:04	ID requested
⏵ 1	2024-11-18 10:40:10	announced

Dataset with its publication pending

submitter keyword: Knockdown, DDX18,SILAC

Jianlong Wang
contact affiliation	Department of Medicine, Columbia University Irving Medical Center
contact email	jw3925@cumc.columbia.edu
lab head
Xin Huang
contact affiliation	Department of Medicine, Columbia University Irving Medical Center
contact email	xh2200@cumc.columbia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD057813

PRIDE project URI

• PRIDE
  1. PXD057813
     1. Label: PRIDE project
     2. Name: SILAC of hESCs with DDX18 knockdown