PXD057805

PXD057805 is an original dataset announced via ProteomeXchange.

Title	Digly analysis of human Kv7.1 transfected in HEK293 cells.
Description	Polyubiquitin chain diversity generates a ‘ubiquitin code’ that universally regulates protein functional expression but functions of polyubiquitin diversity are mostly unknown. Using digly mass spectrometry, we have evaluated the basal polyubiquitin diversity on Kv7.1 ion channels expressed with a C-terminal YFP tag in HEK293 cells. Further, we co-expressed the E3 ligases NEDD4L and ITCH which downregulate Kv7.1 channels and investigated the change in polyubiquitin linkages on the channel.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:08:02.469.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rajesh Soni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-12 09:42:30	ID requested
⏵ 1	2025-07-13 16:08:03	announced

10.1038/s41467-025-60893-0;

Shanmugam SK, Kanner SA, Zou X, Amarh E, Choudhury P, Soni R, Kass RS, Colecraft HM, 7. 1 (KCNQ1) surface expression with engineered linkage-selective deubiquitinases. Nat Commun, 16(1):5805(2025) [pubmed]

submitter keyword: Kv7.1 ion channel, NEDD4L, ITCH, polyubiquitin, protein degradation, ubiquitin code

Henry M. Colecraft
contact affiliation	Interim Chair, Deartment of Molecular Pharmacoloy and Therapeutics John C. Dalton Professor, Deapartment of Physiology and Cellular Biophysics Columbia University College of Physicians and Surgeons
contact email	hc2405@cumc.columbia.edu
lab head
Rajesh Soni
contact affiliation	Columbia University Medical center
contact email	rs3869@cumc.columbia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD057805

PRIDE project URI

• PRIDE
  1. PXD057805
     1. Label: PRIDE project
     2. Name: Digly analysis of human Kv7.1 transfected in HEK293 cells.