PXD057800

PXD057800 is an original dataset announced via ProteomeXchange.

Title	Quantification of intra-amniotic inflammation in late preterm prelabour rupture of membranes
Description	Preterm prelabour rupture of membranes (PPROM) complicated by intra-amniotic inflammation (IAI) represents a substantial portion of preterm birth cases. Currently, IAI is frequently defined as amniotic fluid IL-6 concentration above 2600 pg/mL However, the amniotic fluid IL-6 concentration was never correlated with the global response of other proinflammatory proteins to the ongoing IAI. In this cross-sectional study, protein quantification was performed using mass spectrometry (MS) analysis with isobaric peptide labeling followed by target quantification of selected proinflammatory proteins. Levels of amniotic fluid proteins determined by MS were put into the correlation with IL-6 concentration determined by ECLIA. In total, 925 proteins were efficiently quantified in all samples and differential expression analysis revealed 378 proteins upregulated towards IL-6 concentration above 10 000 pg/mL. Four proteins were selected to verify the achieved results (LCN2, MMP8, MPO, and S100A12). ROC curves were designed and the IL-6 concentration of 10 000 pg/mL was determined as cutoff value for global IAI response.
HostingRepository	PRIDE
AnnounceDate	2025-04-30
AnnouncementXML	Submission_2025-04-30_06:26:47.197.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rudolf Kupcik
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-12 08:29:26	ID requested
⏵ 1	2025-04-30 06:26:51	announced

10.1021/acsomega.4c09377;

Kup, č, í, k R, Len, č, ov, á O, Mazurov, á Y, Š, t, ě, rba M, Vajrychov, á M, LC-MS/MS for Wide-Scale Proteomic Analysis of Anthracycline-Induced Cardiomyopathy. ACS Omega, 10(12):11980-11993(2025) [pubmed]

submitter keyword: intra-amniotic inflammation, late PPROM, amniotic fluid proteome,IL-6, IAI, LC-MS/MS

Marie Vajrychova
contact affiliation	Biomedical Research Centre, University Hospital Hradec Kralove, Sokolska 581, 50005 Hradec Kralove, Czech Republic
contact email	marie.vajrychova@fnhk.cz
lab head
Rudolf Kupcik
contact affiliation	Biomedical Research Centre, University Hospital Hradec Kralove, Hradec Kralove
contact email	rudolf.kupcik@fnhk.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD057800

PRIDE project URI

• PRIDE
  1. PXD057800
     1. Label: PRIDE project
     2. Name: Quantification of intra-amniotic inflammation in late preterm prelabour rupture of membranes