PXD057784

PXD057784 is an original dataset announced via ProteomeXchange.

Title	Study of molecular mechanisms in lung injury due to ultra-high and conventional dose rate pulsed radiation based on 4D-DIA proteomics
Description	Objective: To investigate the mechanism of ultra-high dose rate pulsed radiation in radiation-induced lung injury (RILI), providing an experimental and theoretical basis for the application of FLASH-RT in radiotherapy. Methods: C57BL/6J mice were randomly divided into three groups: a sham group, a FLASH-irradiated group, and a CONV-irradiated group. A whole-body irradiation with a single dose of 3 Gy (200Gy/s for FLASH group and 0.3Gy/s for CONV group) using electron rays was used to establish models of lung injury. After 3 months, lung tissues were stained with HE and Masson stains to observe pathological changes in lung tissue and subjected to 4D-Fast DIA quantitative proteomics, with the sequencing data validated by Western blot. Results: The mice in FLASH group had less lung tissue damage and lower levels of fibrosis compared to the CONV group. Proteomic sequencing showed significant differences in CCT6b protein expression between the two irradiation groups. As verified by the WB assays, the expression level of CCT6b was significantly reduced in the CONV group of mice compared with the SHAM and FLASH groups. With the down-regulation of CCT6b, there was a notable decrease in the expression of E-cadherin, accompanied by an increase in the expression of α-smooth muscle actin and Vimentin. Conclusion: The differential response in the level of lung fibrosis caused by the two types of radiation may be related to the level of CCT6b expression, but the specific mechanism of action needs to be further investigated.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:02:23.138.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ChangZhen Wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-12 03:54:15	ID requested
⏵ 1	2025-05-07 05:02:24	announced

10.1038/s41598-025-87247-6;

Li M, Zhang L, Gao A, Xu J, Wang X, Liu X, Yan D, Zou D, Wu S, Sun B, Dong G, Wang C, Molecular mechanisms of lung injury from ultra high and conventional dose rate pulsed radiation based on 4D DIA proteomics study. Sci Rep, 15(1):5425(2025) [pubmed]

submitter keyword: Radiation-induced lung injury

Ultra-high dose rate

FLASH radiotherapy

fibrosis

FLASH effect

ChangZhen Wang
contact affiliation	Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China
contact email	wangcz2002@aliyun.com
lab head
ChangZhen Wang
contact affiliation	Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China
contact email	wangcz2002@aliyun.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD057784

PRIDE project URI

• PRIDE
  1. PXD057784
     1. Label: PRIDE project
     2. Name: Study of molecular mechanisms in lung injury due to ultra-high and conventional dose rate pulsed radiation based on 4D-DIA proteomics