PXD057778

PXD057778 is an original dataset announced via ProteomeXchange.

Title	Identification of protein substrates of a novel proteolysis targeting chimera, PROTAC124 in tongue squamous cell carcinoma cell line CAL-33 overexpressing Leucine Zipper-bearing Kinase (LZK).
Description	Head and neck squamous cell carcinomas (HNSCC) are aggressive epithelial tumors with a low and unchanged 50% 5-year survival rate. MAP3K13 gene encoding Leucine Zipper-bearing Kinase (LZK) is often amplified in HNSCC patients and can be an oncogenic driver for tumorigenesis. In order to reduce catalytic and non-catalytic functions of LZK we synthetized novel, potent and selective degrader of LZK protein using proteolysis targeting chimera technology. We confirmed efficiency and specificity of our PROTAC by shotgun proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-15_16:08:32.525.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-12 00:49:32	ID requested
⏵ 1	2025-06-15 16:08:33	announced

Katerji M, Bergman KL, Lindberg E, Rubin MR, Afifi M, Funk AL, Woodroofe CC, Nyswaner K, Karpi, ń, ska K, Serwa R, Cappell SD, Marusiak A, Swenson RE, Brognard JF, Discovery of potent and selective PROTACs for the protein kinase LZK for the treatment of head and neck cancer. J Biol Chem, 301(5):108452(2025) [pubmed]

10.1016/j.jbc.2025.108452;

submitter keyword: MAP3K13), head and neck cancer, targeted protein degradation,PROTAC (Proteolysis targeting chimera), HNSCC, Leucine Zipper-bearing Kinase (LZK

Anna Marusiak
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	a.marusiak@imol.edu.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD057778

PRIDE project URI

• PRIDE
  1. PXD057778
     1. Label: PRIDE project
     2. Name: Identification of protein substrates of a novel proteolysis targeting chimera, PROTAC124 in tongue squamous cell carcinoma cell line CAL-33 overexpressing Leucine Zipper-bearing Kinase (LZK).