PXD057760

PXD057760 is an original dataset announced via ProteomeXchange.

Title	Developing a Novel and Integrative Method to Systematically Investigate the Stability of N-Terminal Proteoforms
Description	Protein N-terminal proteoforms, which include both N-terminal modifications and sequence variation arising from processes such as proteolytic cleavage, are common in cells and have been reported to significantly impact protein stability. Systematically examining the correlation between these N-terminal proteoforms and protein stability is essential for understanding their biological roles. Recently, thermal stability analysis has been applied to study protein modifications. Here, we combined Thermal Proteome Profiling (TPP) with tandem mass tag (TMT)-Terminal Amine Isotopic Labeling of Substrates (TAILS) to globally investigate the thermal stability of N-terminal proteoforms in THP-1 cells. The thermal stability of more than 13,000 unique N-terminal peptides corresponding to approximately 4,700 proteins were quantified. Overall, N-terminal proteoforms exhibited higher thermal stability than proteins identified in whole proteome group. A series of factors such as hydrophobicity and secondary structure influenced the thermal stability differences of N-terminal proteoforms.
HostingRepository	PRIDE
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_15:20:45.655.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zeyu Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-11 16:33:30	ID requested
⏵ 1	2025-08-21 15:20:46	announced

Dataset with its publication pending

submitter keyword: thermal proteome profiling, N-terminal acetylation,N-terminome, thermal stability difference, Terminal amine isotopic labeling of substrates, TAILS, TPP, N-terminal proteoform

Ronghu Wu
contact affiliation	School of Chemistry and Biochemistry and the Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, USA
contact email	ronghu.wu@chemistry.gatech.edu
lab head
Zeyu Wang
contact affiliation	Graduate student
contact email	zwang3136@gatech.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057760
     1. Label: PRIDE project
     2. Name: Developing a Novel and Integrative Method to Systematically Investigate the Stability of N-Terminal Proteoforms