PXD057754

PXD057754 is an original dataset announced via ProteomeXchange.

Title	Binding of Glycyl-tRNA synthetase to the 3´UTR stimulates Mengovirus RNA translation
Description	Picornaviruses are small viruses with a plus strand (i.e. mRNA orientation) RNA genome. Due to their limited coding capacity, these viruses use RNA secondary structures in their genomic RNAs like aptamers to bind cellular proteins. The resulting molecular interactions are then used to support translation and replication of the viral RNA. In this study, we have characterized a tRNA anticodon stem-loop-like structure in the 3´ untranslated region (3´UTR) of Mengovirus, a member of the Cardiovirus group in the Picornaviridae family. This RNA structure specifically binds to the cellular enzyme Glycyl-tRNA synthetase (GARS or GlyRS). Mutation of the conserved CCA motif in the loop of this GARS binding element (GBE) as well as deletion of the anticodon binding domain of GARS impair binding. Infection with a Mengovirus carrying a mutated GBE results in reduced replication in HeLa cells and in neuronal cells. Translation of the full length Mengovirus genome in HeLa cells is impaired by the GBE mutation directly after transfection, independent of the activity of the viral polymerase. Consistently, recruitment of translation elongation factors and ribosomes to translation reporter RNAs bearing the Mengovirus untranslated regions and translation efficiencies of these Mengovirus translation reporter RNAs are impaired by mutation of the GBE, while RNA stability is not affected. Thus, this conserved GARS binding element in the Mengovirus 3´UTR is involved in regulation of Mengovirus RNA translation likely on the elongation stage.
HostingRepository	PRIDE
AnnounceDate	2025-12-03
AnnouncementXML	Submission_2025-12-03_08:18:59.480.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uwe Linne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mengo virus; NCBI TaxID: NEWT:12107; scientific name: Viruses; NCBI TaxID: NCBITaxon:10239;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-11 07:49:43	ID requested
⏵ 1	2025-12-03 08:18:59	announced

Dataset with its publication pending

submitter keyword: Picornavirus, Aminoacyl-tRNA-Synthetase, GlyRS, Mengovirus, GBE, Poliovirus, MV, Translation, GARS binding element

Dr. Uwe Linne
contact affiliation	Philipps-Universität Marburg Faculty of Chemistry Hans-Meerwein-Str. 4 35043 Marburg GERMANY
contact email	linneu@staff.uni-marburg.de
lab head
Uwe Linne
contact affiliation	Head of Core Facility Mass Spectrometry, Faculty of Chemistry, Philipps-University Marburg
contact email	linneu@staff.uni-marburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057754
     1. Label: PRIDE project
     2. Name: Binding of Glycyl-tRNA synthetase to the 3´UTR stimulates Mengovirus RNA translation