PXD057750

PXD057750 is an original dataset announced via ProteomeXchange.

Title	Linking the transcriptome to physiology: response of the proteome of Cupriavidus metallidurans to changing metal availability
Description	Cupriavidus metallidurans CH34 is a metal-resistant bacterium. Its metal homeostasis is based on a flow equilibrium of metal ion uptake and efflux reactions, which adapts to changing metal concentrations within an hour. At high metal concentrations, up-regulation of the genes for metal efflux systems occurs within minutes. Here, we investigates the changes in the bacterial proteome accompanying these genetic and physiological events after 1.5 cell duplications, which took 3 hours. To that end, C. metallidurans CH34 and its plasmid-free derivative, AE104, were challenged either with a toxic metal mix or were cultivated under metal-starvation conditions, followed by bottom-up proteomics. When metal-shocked or -starved cells were compared with their respective controls, 3,540 proteins changed in abundance, with 76% appearing in one, but not the other, condition; the remaining 24% were up- or down-regulated. Metal-shocked C. metallidurans strains had adjusted their proteomes to combat metal stress. The most prominent polypeptides were the products of the plasmid-encoded metal-resistance determinants in strain CH34, particularly the CzcCBA transenvelope efflux system. Moreover, the influence of antisense transcripts on the proteome was also revealed. In one specific example, the impact of an asRNA on the abundance of gene products could be demonstrated and this yielded new insights into the function of the transmembrane efflux complex ZniCBunder conditions of metal starvation.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_04:39:49.795.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matt Fuszard
SpeciesList	scientific name: Cupriavidus metallidurans CH34; NCBI TaxID: 266264; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-11 07:32:33	ID requested
⏵ 1	2025-05-07 04:39:50	announced

Galea D, Herzberg M, Dobritzsch D, Fuszard M, Nies DH, Linking the transcriptome to physiology: response of the proteome of Cupriavidus metallidurans to changing metal availability. Metallomics, 16(12):(2024) [pubmed]

10.1093/mtomcs/mfae058;

submitter keyword: Cupriavidus metallidurans, proteomics,Transenvelope efflux systems, Zinc, metal starvation, metal homeostasis

Dietrich H. Nies
contact affiliation	Martin-Luther-Universitaet Halle-Wittenberg, Institute for Biology/Microbiology
contact email	dietrich.nies@mikrobiologie.uni-halle.de
lab head
Matt Fuszard
contact affiliation	Martin-Luther-Universität Halle-Wittenberg, Core Facility — Proteomic Mass Spectrometry
contact email	matthew.fuszard@medizin.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057750
     1. Label: PRIDE project
     2. Name: Linking the transcriptome to physiology: response of the proteome of Cupriavidus metallidurans to changing metal availability