PXD057739

PXD057739 is an original dataset announced via ProteomeXchange.

Title	Lipopolysaccharide stimulates dynamic changes in B cell metabolism to promote cell proliferation - naive and LPS data
Description	Following activation, naïve B cells exit quiescence and enter a proliferative phase before becoming mature antibody-producing or memory B cells. One method of activation is to use Lipopolysaccharide (LPS), a well-established B cell mitogen that can drive proliferation ex vivo. Using quantitative proteomics, we identify an upregulation of proteins following LPS stimulation involved in metabolic pathways including amino acid uptake and cholesterol biosynthesis. We highlight the large amino acid transporter SLC7A5 as a target of LPS-induced activation, and by utilising SLC7A5 conditional knockout mice, we demonstrate the requirement of this transporter for B cell proliferation ex vivo. Inhibition of the rate-limiting enzymes HMG-CoA-reductase (HMGCR) and Squalene monooxygenase in the cholesterol biosynthesis pathway significantly reduced proliferation. The role of the cholesterol biosynthetic pathway was not limited to LPS. Stimulation via TLR7, TLR9, CD40 and the B Cell Receptor also increased cholesterol levels and inhibition of HMGCR reduced proliferation. Together these results suggest a common requirement for metabolic rewiring in B cells to allow proliferation in response to activation.
HostingRepository	PRIDE
AnnounceDate	2026-05-18
AnnouncementXML	Submission_2026-05-18_07:22:43.612.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Howden
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-11 02:49:23	ID requested
⏵ 1	2026-05-18 07:22:44	announced

Dataset with its publication pending

submitter keyword: None

Professor Simon
contact affiliation	School of Life Sciences, University of Dundee, Dundee, UK
contact email	j.s.c.arthur@dundee.ac.uk
lab head
Andrew Howden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057739
     1. Label: PRIDE project
     2. Name: Lipopolysaccharide stimulates dynamic changes in B cell metabolism to promote cell proliferation - naive and LPS data