PXD057699

PXD057699 is an original dataset announced via ProteomeXchange.

Title	Chloroplast ATP-dependent metalloprotease FtsH5/VAR1 confers cold stress tolerance through singlet oxygen and salicylic acid signaling
Description	Cold stress significantly affects plant growth and productivity. Chloroplasts are hotspots of reactive oxygen species (ROS) production and also the cites for the biosynthesis of defense hormone salicylic acid (SA) under environmental stress conditions. However, how plants integrate ROS and SA signals to adapt to stress is poorly understood. Here, we report the Arabidopsis Filamentous temperature-sensitive H5 (FtsH5/VAR1), a thylakoid ATP-dependent zinc metalloprotease, plays an important role in plant cold stress tolerance. The var1-1 mutant displays an albino and retarded growth phenotype under cold stress. We identified a strong correlation of activated SA biosynthesis and signaling with the cold-sensitive phenotype of var1. Reduction of SA accumulation, by overexpressing of salicylate hydroxylase gene (NahG) or knocking out of SA biosynthesis-related genes (ICS1, EDS1, or PAD4), suppressed the albino phenotype of var1. Furthermore, we show that EXECUTOR1 (EX1)-mediated singlet oxygen (1O2) signaling acts upstream of EDS1 to regulate the expression of SA-responsive genes in var1 under cold stress. Importantly, we uncover a moonlighting function of EX2, which the mutation strongly suppresses the cold-sensitive phenotype of var1, in repression the expression of photosynthesis-associated nuclear genes. Together, these results reveal a crucial role of VAR1 in plant cold tolerance and a tight link between 1O2 and SA signaling, corroborating an unheeded function of EX2 in regulating the expression of photosynthesis genes.
HostingRepository	PRIDE
AnnounceDate	2025-05-09
AnnouncementXML	Submission_2025-05-08_22:11:02.256.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yinsong Wang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-09 08:07:54	ID requested
⏵ 1	2025-05-08 22:11:02	announced

10.1016/J.XPLC.2025.101353;

submitter keyword: Arabidopsis, var1, var2,FtsH, cold

Guo-Zhang Wu
contact affiliation	Shanghai Jiao Tong University
contact email	gzwu@sjtu.edu.cn
lab head
Yinsong Wang
contact affiliation	Shanghai Jiao Tong University
contact email	020150910029@sjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057699
     1. Label: PRIDE project
     2. Name: Chloroplast ATP-dependent metalloprotease FtsH5/VAR1 confers cold stress tolerance through singlet oxygen and salicylic acid signaling