PXD057686

PXD057686 is an original dataset announced via ProteomeXchange.

Title	Combined transcriptomic and proteomic analysis of chicken DF-1 cells following innate immune stimulations
Description	The goal of our study was to experimentally quantify both at the transcriptomic and proteomic level the effects of comprehensive innate immune stimulations in chicken. To this end, SILAC-labeled chicken DF-1 cells were mock-treated or treated with either ChIFN1 (1000 IU/mL for 6h), transfected poly (I:C) (4,25 ug/mL for 6 h) or infected with Infectious Bursal Disease Virus strain PBG-98 (IBDV, MOI 5 for 8h). Total cellular RNA was extracted using RNeasy Mini Kit (QIAGEN) and Illumina libraries were prepared and sequenced. In parallel, proteins were extracted and processed for SILAC-based quantitative mass-spectrometry. Samples were run on a LTQ-Velos Orbitrap tandem mass spectrometer and the data processing and quantification were performed by the MaxQuant software.
HostingRepository	PRIDE
AnnounceDate	2026-05-26
AnnouncementXML	Submission_2026-05-26_08:58:00.761.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frederic Sorgeloos
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: NEWT:9031;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-08 15:33:55	ID requested
⏵ 1	2026-05-26 08:58:01	announced

Dataset with its publication pending

submitter keyword: Interferon stimulated genes, ISG, innate immune response, interferon, SILAC,DF-1, chicken, Infectious Bursal Disease, IBDV

Ian Goodfellow
contact affiliation	University of Cambridge
contact email	ig299@cam.ac.uk
lab head
Frederic Sorgeloos
contact affiliation	Institut national de la recherche scientifique
contact email	frederic.sorgeloos@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD057686

PRIDE project URI

• PRIDE
  1. PXD057686
     1. Label: PRIDE project
     2. Name: Combined transcriptomic and proteomic analysis of chicken DF-1 cells following innate immune stimulations