PXD057673

PXD057673 is an original dataset announced via ProteomeXchange.

Title	Mycobacterium abscessus cultured in SCFM with heme treatment.
Description	Mycobacterium abscessus (Mabs) biofilms are altered in the presence of exogenous heme. We wanted to determine how protein levels were affected by heme. Mabs cells were grown in a modified media formulated to mimic the nutritional environment of the sputum of cystic fibrosis (CF) patients. Mabs cells grown in synthetic CF media (SCFM) typically form submerged biofilms, exogenous heme treatment however prevents this film formation and supports growth of a pellicle at the air-liquid interface. Heme treatment reduced several iron uptake and homeostasis proteins, suggesting heme is acting as an iron source for these bacteria. In addition, multiple proteins were upregulated in heme treatment, however, the function of these proteins remains to be determined.
HostingRepository	PRIDE
AnnounceDate	2025-02-23
AnnouncementXML	Submission_2025-02-23_07:20:56.607.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rajesh Soni
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Mycobacterium abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543); NCBI TaxID: 561007;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-08 08:30:23	ID requested
⏵ 1	2025-02-23 07:20:57	announced

10.1128/SPECTRUM.02415-24;

submitter keyword: heme, Mycobacterium abscessus

Rebecca Donegan
contact affiliation	Assistant Professor Chemistry, Barnard College
contact email	rdonegan@barnard.edu
lab head
Rajesh Soni
contact affiliation	Columbia University Medical center
contact email	rs3869@cumc.columbia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD057673

PRIDE project URI

• PRIDE
  1. PXD057673
     1. Label: PRIDE project
     2. Name: Mycobacterium abscessus cultured in SCFM with heme treatment.