PXD057672

PXD057672 is an original dataset announced via ProteomeXchange.

Title	A. aphrophilus uses a type VI secretion system to modify epithelial cell responses to highly leukotoxic A. actinomycetemcomitans
Description	The Type VI secretion system (T6SS) is a bacterial mechanism that delivers effectors to eliminate competitors and modulate host responses. We previously discovered that the oral symbiont Aggregatibacter aphrophilus uses its T6SS to kill the pathobiont Aggregatibacter actinomycetemcomitans. To understand how the T6SS-dependent mechanism of A. aphrophilus affects host cells and A. actinomycetemcomitans, we co-cultured our immortalised epithelial cell line with A. actinomycetemcomitans and various strains of A. aphrophilus: wild-type (HK83 + JP2, n = 6), a T6SS-deficient strain (HK83 Δhcp + JP2, n = 6), and double mutants (HK83 Δtle5 Δglh + JP2, n = 6). As a baseline, we also examined single inoculations of A. aphrophilus (HK83, n = 6), the Δhcp mutant (HK83 Δhcp, n = 6), the Δtle5 Δglh mutant (HK83 Δtle5 Δglh, n = 6), and A. actinomycetemcomitans JP2 (JP2, n = 6). Cells not challenged served as controls. This study aims to identify specific effectors and characterize host responses to A. aphrophilus T6SS.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_16:52:22.899.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Bao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	methionine oxidation with neutral loss of 64 Da; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-08 07:52:39	ID requested
⏵ 1	2025-11-09 16:52:23	announced

10.1177/00220345251337745;

Bao K, Oscarsson J, Gehring P, Grossmann J, Belibasakis GN, Bostanci N, T6SS Effectors in Host-Bacterial Interactions. J Dent Res, 104(13):1487-1494(2025) [pubmed]

submitter keyword: A. aphrophilus, A. actinomycetemcomitans, T6SS

Nagihan Bostanci
contact affiliation	Department of Dental Medicine (DENTMED), OF, Karolinska Institutet
contact email	nagihan.bostanci@ki.se
lab head
Kai Bao
contact affiliation	postdoctoral researcher
contact email	kai.bao@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057672
     1. Label: PRIDE project
     2. Name: A. aphrophilus uses a type VI secretion system to modify epithelial cell responses to highly leukotoxic A. actinomycetemcomitans