PXD057669

PXD057669 is an original dataset announced via ProteomeXchange.

Title	Decoding the impact of interspecies interactions on biofilm matrix components
Description	Multispecies biofilms consist of complex communities where extracellular polymeric substances (EPS) are vital in their structure, adaptability, and function. However, characterizing the components of EPS, particularly glycans and proteins, remains a challenge due to the diverse species and their interactions within the matrix. This study examined how interactions between different species affect EPS components' production and spatial organization. We utilized a consortium of four bacterial soil isolates that have previously demonstrated various intrinsic properties in biofilm communities: Microbacterium oxydans, Paenibacillus amylolyticus, Stenotrophomonas rhizophila, and Xanthomonas retroflexus. We used fluorescence lectin-binding analysis (FLBA) to identify specific glycan components and meta-proteomics to characterize matrix proteins in mono- and multispecies biofilms. Our results revealed diverse glycan structures and compositions, including fucose and different amino sugar-containing polymers, with substantial differences between monospecies and multispecies biofilms. In isolation, M. oxydans produced galactose/N-Acetylgalactosamine network-like structures and influenced the matrix composition in multispecies biofilms. Proteomic analysis revealed flagellin proteins in Xanthomonas and Paenibacillus, particularly in multispecies biofilms. Additionally, surface-layer proteins and a unique peroxidase were found in P. amylolyticus multispecies biofilms, indicating enhanced oxidative stress resistance and structural stability under these conditions. This study highlights the crucial role of interspecies interactions in shaping biofilm matrices and the production of glycans and proteins. These findings deepen our understanding of biofilm complexity and may lead to new approaches for controlling biofilms in various environments.
HostingRepository	PRIDE
AnnounceDate	2025-03-31
AnnouncementXML	Submission_2025-03-31_02:07:58.695.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristina Amador
SpeciesList	scientific name: Paenibacillus amylolyticus; NCBI TaxID: 1451; scientific name: Microbacterium oxydans; NCBI TaxID: 82380; scientific name: Stenotrophomonas rhizophila; NCBI TaxID: 216778; scientific name: Xanthomonas retroflexus; NCBI TaxID: 305959;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-08 06:41:16	ID requested
⏵ 1	2025-03-31 02:07:59	announced

10.1016/J.BIOFLM.2025.100271;

submitter keyword: EPS,Multispecies biofilms, matrix proteomics

Cristina Amador
contact affiliation	Section of Microbiology Department of Biology University of Copenhagen Copenhagen, Denmark
contact email	chierro@bio.ku.dk
lab head
Cristina Amador
contact affiliation	Copenhagen University
contact email	chierro@bio.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD057669

PRIDE project URI

• PRIDE
  1. PXD057669
     1. Label: PRIDE project
     2. Name: Decoding the impact of interspecies interactions on biofilm matrix components