PXD057662

PXD057662 is an original dataset announced via ProteomeXchange.

Title	Toxoplasma Tepsin is essential for transport from the trans-Golgi to the vacuole
Description	Apicomplexans are intracellular parasites that invade and replicate within host cells. During their adaptation to this parasitic life-style these protozoans evolved numerous new organelles. Paradoxically, these parasites lost several cellular machineries which are conserved in other eukaryotes, including components involved in vesicular transport and the cytoskeleton. While some genes have been lost during evolution others might not be identifiable using bioinformatic approaches. We previously performed a phenotypic screen to determine the function of essential genes, only found in apicomplexan parasites and identified a hypothetical protein (TGGT1_301410). Here we demonstrate that this protein is a distant orthologue of Tepsin (TgTEP), an accessory protein for the adaptor complex 4 (AP4). TgTEP localises to the trans-Golgi, where it is required for the transport of material to the plant-like vacuole (PLVAC) of the parasite. Interestingly, TgTEP interacts with the AP4 and clathrin.
HostingRepository	PRIDE
AnnounceDate	2025-10-14
AnnouncementXML	Submission_2025-10-14_00:39:57.830.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: NEWT:383379;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-08 04:09:57	ID requested
⏵ 1	2025-10-14 00:39:58	announced

10.1083/JCB.202312109;

submitter keyword: Toxoplasma gondii, tepsin, vesicular trafficking, adaptor protein 4

Prof. Dr. rer. nat. Markus Meissner
contact affiliation	Department of Experimental Parasitology LMU München Lena-Christ-Str. 48 82152 Planegg-Martinsried Telefon: +49 (0) 89 2180 - 3622 Fax: +49 (0) 89 2180 - 3623 E-Mail: markus.meissner@lmu.de
contact email	Markus.Meissner@lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD057662

PRIDE project URI

• PRIDE
  1. PXD057662
     1. Label: PRIDE project
     2. Name: Toxoplasma Tepsin is essential for transport from the trans-Golgi to the vacuole