PXD057655

PXD057655 is an original dataset announced via ProteomeXchange.

Title	Proteomics to reveal relationship between menstrual cycle and subjective conditions: a pilot study
Description	The menstrual cycle is a vital aspect of women’s health, influencing both physical and mental well-being. Many women experience premenstrual symptom (PMS), such as stomachache and irritability, which can impact daily life. However, the underlying biological mechanisms remain poorly understood, indicating a need for further research. This study aimed to explore the relationship between proteins and subjective condition related to menstrual cycle using two-dimensional gel electrophoresis (2-DE) technique. Serum samples were collected weekly from 42 female students over a period of 5 weeks, resulting in a total of 210 2-DE images. At the same time, two female hormones -- estradiol and progesterone -- were measured. On the morning of every blood collection day, participants rated their subjective condition (SC) score on a scale of 1-5, with scores closer to 5 indicating better condition. These data were categorized into three groups: scores of 1-2 as SC Low, a score of 3 as SC Mid, and scores of 4-5 as SC High. The Steel-Dwass test comparing these groups revealed that two proteins related to oxidative stress, kallistatin and transferrin, are significantly higher in the SC high group compared to both the SC Low and SC Mid groups. Although the specific symptoms that primarily affect SC are not known, oxidative stress is believed to be associated with fatigue and migraine. This study demonstrates that proteins linked to oxidative stress reflect women’s subjective condition during the menstrual cycle. Notably, the evaluation of discernment ability using receiver operating characteristic curve (ROC) shows kallistatin and transferrin exhibit greater discernment than conventional hormonal markers for identifying negative or positive conditions. Investigating well-being related to the menstrual cycle through the lens of proteomics proves to be valuable approach, and targeting these proteins may lead to effective health management strategies for women.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_16:08:33.158.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD057655
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kai Fushimi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-08 01:45:38	ID requested
⏵ 1	2026-06-14 16:08:33	announced

10.6019/PXD057655;

10.1038/s41598-026-47566-8;

Fushimi K, Kondo T, Ikegami N, Yamada M, Wong SY, Sakamaki-Sunaga M, Hayashi N, Proteomics to reveal relationship between menstrual cycle and subjective conditions. Sci Rep, 16(1):(2026) [pubmed]

submitter keyword: None

Kai Fushimi
contact affiliation	School of Life Science and Technology, Institute of Science Tokyo
contact email	fushimi.k.2b47@m.titech.ac.jp
lab head
Kai Fushimi
contact affiliation	Tokyo Institute of Technology, Grd of Life science and technology
contact email	fushimi.k.2b47@m.isct.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD057655

PRIDE project URI

• PRIDE
  1. PXD057655
     1. Label: PRIDE project
     2. Name: Proteomics to reveal relationship between menstrual cycle and subjective conditions: a pilot study