PXD057610

PXD057610 is an original dataset announced via ProteomeXchange.

Title	Evolutionary adaptations of doublet microtubules in trypanosomatid parasites
Description	The movement and pathogenicity of trypanosomatid species, the causative agents of trypanosomiasis and leishmaniasis, are dependent on a flagellum containing an axoneme of dynein-bound doublet microtubules (DMTs). Here, we present cryo-EM structures of DMTs from two trypanosomatid species, Leishmania tarentolae and Crithidia fasciculata, at resolutions up to 2.7 Å. The structures reveal 27 trypanosomatid-specific microtubule inner proteins, a specialized dynein-docking complex, and the presence of paralogous proteins that enable higher-order periodicities or proximal-distal patterning. Leveraging the genetic tractability of trypanosomatid species, we quantify the location and contribution of each structure-identified protein to swimming behavior. Our study shows that proper B-tubule closure is critical for flagellar motility, exemplifying how integrating structural identification with systematic gene knockout can dissect individual protein contributions to flagellar motility.
HostingRepository	PRIDE
AnnounceDate	2025-03-04
AnnouncementXML	Submission_2025-03-04_11:50:09.295.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophe Alvarez
SpeciesList	scientific name: Crithidia fasciculata; NCBI TaxID: 5656;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-07 02:26:12	ID requested
⏵ 1	2025-03-04 11:50:09	announced

10.1126/SCIENCE.ADR5507;

submitter keyword: doublet microtubules, flagellar motility, trypanosomatid

Sophie Alvarez
contact affiliation	Proteomics and Metabolomics Facility, University of Nebraska- Lincoln
contact email	salvarez@unl.edu
lab head
Sophe Alvarez
contact affiliation	University of Nebraska - Lincoln
contact email	salvarez@unl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD057610

PRIDE project URI

• PRIDE
  1. PXD057610
     1. Label: PRIDE project
     2. Name: Evolutionary adaptations of doublet microtubules in trypanosomatid parasites