PXD057599

PXD057599 is an original dataset announced via ProteomeXchange.

Title	Ribosome biogenesis in plants reqRibosome biogenesis in plants requires the nuclear envelope and mitochondria localized OPENER complexuires the nuclear envelope and mitochondria localized OPENER complex
Description	Eukaryotic ribosome biogenesis proceeds from nucleolus to cytosol assisted by various assembly factors. The process is evolutionarily conserved across eukaryotes but differences between kingdoms are emerging. Here, we describe how the OPENER (OPNR) protein complex is required for 60S ribosome assembly in the model plant Arabidopsis thaliana. The complex was observed on both nuclear envelope and mitochondria, and contains OPNR, OPENER ASSOCIATED PROTEIN 1 (OAP1), OAP2, Cell Division Cycle 48 D (CDC48D) and Calmodulin-interacting protein 111 (CIP111). Depletion of the OPNR complex components resulted in reproductive lethality and cytoplasmic retention of assembly factors on 60S ribosomes. Subsequent biochemical analyses and structural modelling suggested that OPNR, OAP1 and OAP2 form a claw-like trimer which grabs the ribosome assembly factor RPL24C on the pre-60S ribosome. Our results reveal previously unrecognised subcellular complexity of ribosome biogenesis in plants, and point to mitochondria association as a feature to ensure sufficient translational capacity.
HostingRepository	PRIDE
AnnounceDate	2025-07-24
AnnouncementXML	Submission_2025-07-24_05:53:22.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaochun Zhu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-06 23:47:18	ID requested
⏵ 1	2025-07-24 05:53:23	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana,ribosome, mitochondria, OPNR

Totte Niittylä
contact affiliation	Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183 Umeå, Sweden
contact email	totte.niittyla@slu.se
lab head
Shaochun Zhu
contact affiliation	Umea University
contact email	shaochunzhu@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057599
     1. Label: PRIDE project
     2. Name: Ribosome biogenesis in plants reqRibosome biogenesis in plants requires the nuclear envelope and mitochondria localized OPENER complexuires the nuclear envelope and mitochondria localized OPENER complex