PXD057589

PXD057589 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Baculoviral IAP repeat-containing protein 6 Ubiquitinates KRAS4A
Description	Transcripts of the KRAS locus are alternatively spliced to generate two proteins, KRAS4A and KRAS4B, that differ in their membrane targeting sequences. These splice variants have been conserved for more than 450 million years, suggesting non-overlapping functions driven by differential membrane association. Here we use proximity labeling to map the differential interactomes of the KRAS splice variants. We found 24 and 10 proteins that interact specifically with KRAS4A or KRAS4B, respectively. The KRAS interacting protein most specific to KRAS4A was BIRC6, a large member of the inhibitor of apoptosis protein family unique in possessing E2/E3 ubiquitin ligase activity. We found that this interaction takes place on the Golgi apparatus and results in the mono- and di-ubiquitination of KRAS4A at lysines 128 and 147. Silencing BIRC6 diminished GTP-loading of and growth stimulation by KRAS4A but not KRAS4B. Thus, BIRC6 is a ubiquitin ligase that inhibits apoptosis and also modifies KRAS4A. The mass spectrometry files for figures 1D, 1E, S1B, S1C, Supplemental figure 4A are included here.
HostingRepository	MassIVE
AnnounceDate	2024-11-06
AnnouncementXML	Submission_2024-11-06_16:14:35.358.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Beatrix Ueberheide
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; GG
Instrument	Q Exactive; Orbitrap Eclipse; timsTOF HT

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-06 16:06:30	ID requested
⏵ 1	2024-11-06 16:14:35	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: KRAS4A, proximity labeling, BIRC6, DatasetType:Proteomics

submitter keyword: KRAS4A, proximity labeling, BIRC6, DatasetType:Proteomics

Contact List

Beatrix Ueberheide
contact affiliation	NYU Langone Grossman School of Medicine
contact email	beatrix.ueberheide@nyulangone.org
lab head
Beatrix Ueberheide
contact affiliation	NYU School of Medicine
contact email	beatrixueberheide@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000096322/