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PXD057576

PXD057576 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome changes associated with effect of high-dose single-fractionation radiation on lung adenocarcinoma cell lines
DescriptionLung cancer is a leading cause of cancer-related mortality globally, with non-small cell lung cancer (NSCLC) representing 85% of cases. Advances in treatment modalities, including the emergence of antibody-drug conjugates and stereotactic radiation therapy, have improved outcomes. However, the possible synergistic effects of these therapies remain underexplored at the molecular level. This study investigated high-dose radiation-induced proteomic changes in lung adenocarcinoma cell line HCC-44 grown adherently and cell line A549, grown as adherent cells and 3D spheroids. Our hypothesis was that proteins upregulated by 10 Gy irradiation serve as resistance drivers in cancerous cells and can thus represent potential therapeutic targets. The label-free mass spectrometry revealed distinct proteomic responses to 10 Gy irradiation, varying by cell line and culture conditions. Differentially expressed proteins elevated in the irradiated samples included ephrin type-A receptor 2 (EPHA2) in adherent cells and insulin-like growth factor 2 receptor (IGF2R), tetraspanin 3 (TSPAN3) as well as cathepsin D (CTSD) in spheroids. The validation of these targets was carried out via Western blot, immunofluorescence, viability assay and spheroid formation assay. The functional assays demonstrated that irradiation sensitized A549 cells to EPHA2 and CTSD inhibitors. These findings underscore the potential of integrating radiation and targeted therapies in NSCLC treatment, and highlight EPHA2 as a promising candidate for future therapeutic strategies.
HostingRepositoryPRIDE
AnnounceDate2025-07-06
AnnouncementXMLSubmission_2025-07-06_06:44:28.201.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD057576
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDarja Lavogina
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-11-06 05:41:46ID requested
12025-07-06 06:44:28announced
Publication List
10.6019/PXD057576;
10.1038/S41598-025-09285-4;
Keyword List
submitter keyword: proteomics, Ephrin Type-A Receptor 2, high-dose radiation,Lung adenocarcinoma, 3D cell culture
Contact List
Jana Jaal
contact affiliationInstitute of Clinical Medicine, University of Tartu, Tartu, Estonia
contact emailjana.jaal@ut.ee
lab head
Darja Lavogina
contact affiliationUniversity of Tartu
contact emaildarja.lavogina@ut.ee
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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