PXD057576 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome changes associated with effect of high-dose single-fractionation radiation on lung adenocarcinoma cell lines |
Description | Lung cancer is a leading cause of cancer-related mortality globally, with non-small cell lung cancer (NSCLC) representing 85% of cases. Advances in treatment modalities, including the emergence of antibody-drug conjugates and stereotactic radiation therapy, have improved outcomes. However, the possible synergistic effects of these therapies remain underexplored at the molecular level. This study investigated high-dose radiation-induced proteomic changes in lung adenocarcinoma cell line HCC-44 grown adherently and cell line A549, grown as adherent cells and 3D spheroids. Our hypothesis was that proteins upregulated by 10 Gy irradiation serve as resistance drivers in cancerous cells and can thus represent potential therapeutic targets.
The label-free mass spectrometry revealed distinct proteomic responses to 10 Gy irradiation, varying by cell line and culture conditions. Differentially expressed proteins elevated in the irradiated samples included ephrin type-A receptor 2 (EPHA2) in adherent cells and insulin-like growth factor 2 receptor (IGF2R), tetraspanin 3 (TSPAN3) as well as cathepsin D (CTSD) in spheroids. The validation of these targets was carried out via Western blot, immunofluorescence, viability assay and spheroid formation assay. The functional assays demonstrated that irradiation sensitized A549 cells to EPHA2 and CTSD inhibitors. These findings underscore the potential of integrating radiation and targeted therapies in NSCLC treatment, and highlight EPHA2 as a promising candidate for future therapeutic strategies. |
HostingRepository | PRIDE |
AnnounceDate | 2025-07-06 |
AnnouncementXML | Submission_2025-07-06_06:44:28.201.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD057576 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Darja Lavogina |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-11-06 05:41:46 | ID requested | |
⏵ 1 | 2025-07-06 06:44:28 | announced | |
Publication List
Keyword List
submitter keyword: proteomics, Ephrin Type-A Receptor 2, high-dose radiation,Lung adenocarcinoma, 3D cell culture |
Contact List
Jana Jaal |
contact affiliation | Institute of Clinical Medicine, University of Tartu, Tartu, Estonia |
contact email | jana.jaal@ut.ee |
lab head | |
Darja Lavogina |
contact affiliation | University of Tartu |
contact email | darja.lavogina@ut.ee |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD057576 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD057576
- Label: PRIDE project
- Name: Proteome changes associated with effect of high-dose single-fractionation radiation on lung adenocarcinoma cell lines