PXD057573

PXD057573 is an original dataset announced via ProteomeXchange.

Title	Translation of bi-directional transcripts generates peptides for the MHC-I pathway.
Description	Antisense transcripts play an important role in generating regulatory non-coding RNAs but if these transcripts are also translated and for what purpose is yet poorly understood. Isolation of polysome fractions followed by RNA sequencing and mass spectrometry analysis of a six frame data-base revealed nascent peptides originating from different reading frames of bi-directional transcripts. Two peptides originating from the antisense strand stimulated the proliferation of CD8+ T cells when presented to peripheral blood mononuclear cells (PBMCs) from nine healthy donors. An antigenic peptide derived from the reverse strand of two cDNA constructs was presented on MHC-I molecules and induced CD8+ T cell activation. These results demonstrate that three frame translation of bi-directional transcripts generate antigenic peptide substrates for the immune system. This discovery holds significance for better understanding the origin of self-discriminating peptide substrates for the MHC-I pathway and for enhancing immune-based therapies against infected or transformed cells.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_04:30:59.321.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lenka Hernychova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-06 04:22:29	ID requested
⏵ 1	2025-05-07 04:31:00	announced

Zavadil F, Henek T, Habault J, Chemali R, Tovar-Fernandez MC, Daskalogianni C, Malbert-Colas L, Wang L, Gnanasundram SV, Vojtesek B, Hernychova L, Apcher S, Fahraeus R, Translation of bi-directional transcripts enhances MHC-I peptide diversity. Front Immunol, 16():1554561(2025) [pubmed]

10.3389/fimmu.2025.1554561;

submitter keyword: Human, mass spectrometry, polysomes, MHC-I pathway

Robin Fahraeus
contact affiliation	French Institute of Health and Medical Research | Inserm · IGM
contact email	robin.fahraeus@inserm.fr
lab head
Lenka Hernychova
contact affiliation	Masaryk Memorial Cancer Institutre, Zluty kopec 7, Brno 656 53, Czech Republic
contact email	lenka.hernychova@mou.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD057573

PRIDE project URI

• PRIDE
  1. PXD057573
     1. Label: PRIDE project
     2. Name: Translation of bi-directional transcripts generates peptides for the MHC-I pathway.