PXD057568 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS analysis of immunoprecipitation samples of a Tetrahymena TFIIB-like protein Emit3 and the zinc-ribbon mutated Emit3 |
Description | Transposable elements (TEs) are threats to genome stability and thus small RNA-mediated heterochromatinization suppresses the transcription of TEs. Paradoxically, transcription of non-coding RNA (ncRNA) from TEs is needed for the production of TE-targeting small RNAs and/or recruiting the silencing machinery to TEs. Hence, specialized RNA polymerase II (Pol II) transcription machinery is required for such unconventional transcription in different organisms. Indeed, the developmental stage-specific Mediator complex (Med)-associated proteins play essential roles in the ncRNA transcription from TE-related sequences in Tetrahymena. Yet it remains unknown how those Med-associated proteins are linked to the TE transcription by Pol II. Here, have found that Pol II is regulated by Emit3, a stage-specific, TFIIB-like protein specialized in TE transcription. Hence, to find out potential interactions, we did LC-MS/MS analysis for immunoprecipitation samples of Emit3 and the zinc-ribbon mutated Emit3. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-13 |
AnnouncementXML | Submission_2025-05-12_19:29:29.858.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Miao TIAN |
SpeciesList | scientific name: Tetrahymena thermophila; NCBI TaxID: 5911; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-11-06 02:52:59 | ID requested | |
⏵ 1 | 2025-05-12 19:29:30 | announced | |
Publication List
Keyword List
submitter keyword: Tetrahymena thermophila, Immunoprecipitation, meiosis, ncRNA transcription, LC-MS/MS |
Contact List
Miao TIAN |
contact affiliation | MOE Key Laboratory of Evolution & Marine Biodiversity and Institute of Evolution & Marine Biodiversity, Ocean University of China |
contact email | miao.tian@ouc.edu.cn |
lab head | |
Miao TIAN |
contact affiliation | Ocean University of China |
contact email | miao.tian@ouc.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD057568
- Label: PRIDE project
- Name: LC-MS/MS analysis of immunoprecipitation samples of a Tetrahymena TFIIB-like protein Emit3 and the zinc-ribbon mutated Emit3