⮝ Full datasets listing

PXD057568

PXD057568 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS analysis of immunoprecipitation samples of a Tetrahymena TFIIB-like protein Emit3 and the zinc-ribbon mutated Emit3
DescriptionTransposable elements (TEs) are threats to genome stability and thus small RNA-mediated heterochromatinization suppresses the transcription of TEs. Paradoxically, transcription of non-coding RNA (ncRNA) from TEs is needed for the production of TE-targeting small RNAs and/or recruiting the silencing machinery to TEs. Hence, specialized RNA polymerase II (Pol II) transcription machinery is required for such unconventional transcription in different organisms. Indeed, the developmental stage-specific Mediator complex (Med)-associated proteins play essential roles in the ncRNA transcription from TE-related sequences in Tetrahymena. Yet it remains unknown how those Med-associated proteins are linked to the TE transcription by Pol II. Here, have found that Pol II is regulated by Emit3, a stage-specific, TFIIB-like protein specialized in TE transcription. Hence, to find out potential interactions, we did LC-MS/MS analysis for immunoprecipitation samples of Emit3 and the zinc-ribbon mutated Emit3.
HostingRepositoryPRIDE
AnnounceDate2025-05-13
AnnouncementXMLSubmission_2025-05-12_19:29:29.858.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMiao TIAN
SpeciesList scientific name: Tetrahymena thermophila; NCBI TaxID: 5911;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-11-06 02:52:59ID requested
12025-05-12 19:29:30announced
Publication List
10.1093/NAR/GKAF427;
Keyword List
submitter keyword: Tetrahymena thermophila, Immunoprecipitation, meiosis, ncRNA transcription, LC-MS/MS
Contact List
Miao TIAN
contact affiliationMOE Key Laboratory of Evolution & Marine Biodiversity and Institute of Evolution & Marine Biodiversity, Ocean University of China
contact emailmiao.tian@ouc.edu.cn
lab head
Miao TIAN
contact affiliationOcean University of China
contact emailmiao.tian@ouc.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD057568
PRIDE project URI
Repository Record List
[ + ]