PXD057556

PXD057556 is an original dataset announced via ProteomeXchange.

Title	An antibody biomarker associated with Onchocerca volvulus microfilariae identified by proteomics of parasite tissue isolated by laser capture microdissection
Description	Onchocerciasis (river blindness), a neglected tropical disease caused by the filarial nematode Onchocerca volvulus, impacts millions of people in sub-Saharan Africa. The World Health Organization coordinates global efforts to eliminate onchocerciasis and has prioritized development of improved diagnostic tests to aid these efforts. Here, we used laser capture microdissection to isolate distinct tissues from histologic sections of O. volvulus worms in subcutaneous nodules removed from onchocerciasis patients. Proteomic analysis identified 2,512 O. volvulus proteins in those tissues, including 264 found only in the microfilariae. From this pool of diagnostic candidates, we selected the putative cuticular collagen OVOC12404 for further study because of its abundance and lack of close homologues in other filarial species. The antigen was localized to the surface of coiled, stretched and tissue microfilariae. IgG antibodies to OVOC12404 were detected by ELISA in plasma from 132 of 160 (81%) onchocerciasis patients. ELISA specificity was 98% based on 42 samples from lymphatic filariasis patients from areas without co-endemic onchocerciasis. In contrast to antibodies to OV16, antibodies to OVOC12404 declined after treatments that cleared O. volvulus microfilariae from the skin. This study showed that proteomic analysis of parasite tissues recovered from histological sections can be used to identify diagnostic targets. Further studies are needed to assess the potential value of an OVOC12404 antibody test as an additional diagnostic tool to support the global program to eliminate onchocerciasis.
HostingRepository	PRIDE
AnnounceDate	2026-07-27
AnnouncementXML	Submission_2026-07-27_03:25:11.015.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD057556
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Byoung-Kyu Cho
SpeciesList	scientific name: Onchocerca sp. NP-2011; NCBI TaxID: NEWT:1079921; scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-05 16:25:40	ID requested
⏵ 1	2026-07-27 03:25:11	announced

10.6019/PXD057556;

Greene SE, Fischer K, Di Maggio LS, Rosa BA, Huang Y, Diekmann I, Cho BK, Lukowski J, Goo YA, Mitreva M, Opoku N, Weil GJ, Fischer PU, An Antibody Biomarker Associated with Onchocerca volvulus Microfilariae Identified by Proteomic Analysis of Parasite Tissues Isolated from Paraffin Embedded O. volvulus Nodules by Laser Capture Microdissection. Am J Trop Med Hyg, 113(2):324-333(2025) [pubmed]

10.4269/ajtmh.24-0793;

submitter keyword: LC-MS/MS, Laser capture microdissection

Young Ah
contact affiliation	Washington University in St. Louis
contact email	ygoo@wustl.edu
lab head
Byoung-Kyu Cho
contact affiliation	Washington University in St. Louis
contact email	byoung-kyu@wustl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057556
     1. Label: PRIDE project
     2. Name: An antibody biomarker associated with Onchocerca volvulus microfilariae identified by proteomics of parasite tissue isolated by laser capture microdissection