PXD057533

PXD057533 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Role of the internal disulfide bond in altering the binding properties of SARS-CoV-2 membrane binding peptide to lipid bilayer
Description	The SARS-CoV-2 spike protein’s membrane-binding domain bridges the viral and host cell membrane, a critical step in triggering membrane fusion. we investigate how the SARS-CoV-2 spike protein interacts with host cell membranes, focusing on a membrane-binding peptide (MBP) located near the TMPRSS2 cleavage site. We observe that the disulfide bridge stabilizes the MBP’s interaction with the membrane, suggesting a structural role in viral entry.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_04:27:38.699.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057533
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Alsteens
SpeciesList	scientific name: Coronaviridae sp.; NCBI TaxID: 1986198;
ModificationList	disulfide crosslinked residues
Instrument	Synapt MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-05 06:04:54	ID requested
⏵ 1	2025-05-07 04:27:39	announced

Publication List

10.6019/PXD057533;
10.1038/s41467-024-55358-9;
Zhang Q, Rosa RSL, Ray A, Durlet K, Dorrazehi GM, Bernardi RC, Alsteens D, Probing SARS-CoV-2 membrane binding peptide via single-molecule AFM-based force spectroscopy. Nat Commun, 16(1):6(2025) [pubmed]

10.6019/PXD057533;

10.1038/s41467-024-55358-9;

Zhang Q, Rosa RSL, Ray A, Durlet K, Dorrazehi GM, Bernardi RC, Alsteens D, Probing SARS-CoV-2 membrane binding peptide via single-molecule AFM-based force spectroscopy. Nat Commun, 16(1):6(2025) [pubmed]

Keyword List

submitter keyword: Membrane binding peptide, SARS-CoV-2, Cholesterol, disulfide bond

submitter keyword: Membrane binding peptide, SARS-CoV-2, Cholesterol, disulfide bond

Contact List

David Alsteens
contact affiliation	Université Catholique de Louvain, Louvain Institute of Biomolecular Science and Technology
contact email	david.alsteens@uclouvain.be
lab head
David Alsteens
contact affiliation	UCLouvain
contact email	david.alsteens@uclouvain.be
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD057533

PRIDE project URI

Repository Record List

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